The cellular basis of adjuvant arthritis. I. Enhancement of cell-mediated passive transfer by concanavalin A and by immunosuppressive pretreatment of the recipient

Joel D. Taurog, Gregory P. Sandberg, Maren L Mahowald

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87 Scopus citations

Abstract

Two reliable systems for the cell-mediated passive transfer of adjuvant arthritis have been developed. Donor rats were sensitized with Mycobacterium butyricum in mineral oil. In the first system, intravenous injection of adjuvant-sensitized donor lymph node or spleen cells into adultthymectomized, lethally irradiated, bone marrow cell-reconstituted syngeneic rats induced arthritis in the recipients. In the second system, adjuvant-sensitized donor lymph node or spleen cells were cultured in vitro with concanavalin A; these cells induced arthritis in normal recipients as well as in thymectomized, irradiated, bone marrow cell-reconstituted recipients. The passively transferred disease in both systems resembled classical adjuvant-induced arthritis clinically, radiographically, and histologically. Neither irradiated, adjuvant-sensitized donor cells nor cells from donors not injected with complete adjuvant could passively transfer arthritis.

Original languageEnglish (US)
Pages (from-to)271-282
Number of pages12
JournalCellular Immunology
Volume75
Issue number2
DOIs
StatePublished - Feb 1 1983

Bibliographical note

Funding Information:
’ This work was supported by Young Investigator Award AM29799-01 from the National Institutes of Health to Dr. Taurog; and by grants from the Minnesota Chapter, Arthritis Foundation; the Minnesota Medical Foundation; the Minnesota Chapter, Lupus Foundation of America; the Graduate School of the University of Minnesota; and the Medical School of the University of Minnesota. * To whom correspondence should be addressed.

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