The Cav 1.2 Ca2+ channel is expressed in sarcolemma of type I and IIA myofibers of adult skeletal muscle

Dušan M. Jeftinija, Bo Wang Qing, Sadie L. Hebert, Christopher M. Norris, Zhen Yan, Mark M. Rich, Susan D. Kraner

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Although Ca2+-dependent signaling pathways are important for skeletal muscle plasticity, the sources of Ca2+ that activate these signaling pathways are not completely understood. Influx of Ca2+ through surface membrane Ca2+ channels may activate these pathways. We examined expression of two L-type Ca2+ channels in adult skeletal muscle, the CaV 1.1 and CaV 1.2, with isoform-specific antibodies in Western blots and immunocytochemistry assays. Consistent with a large body of work, expression of the CaV 1.1 was restricted to skeletal muscle where it was expressed in T-tubules. CaV 1.2 was also expressed in skeletal muscle, in the sarcolemma of type I and IIa myofibers. Exercise-induced alterations in muscle fiber types cause a concomitant increase in the number of both CaV 1.2 and type IIa-positive fibers. Taken together, these data suggest that the CaV 1.2 Ca2+ channel is expressed in adult skeletal muscle in a fiber type-specific manner, which may help to maintain oxidative muscle phenotype.

Original languageEnglish (US)
Pages (from-to)482-490
Number of pages9
JournalMuscle and Nerve
Volume36
Issue number4
DOIs
StatePublished - Oct 1 2007

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Sarcolemma
Skeletal Muscle
Muscles
Ion Channels
Protein Isoforms
Western Blotting
Immunohistochemistry
Phenotype
Antibodies

Keywords

  • Calcineurin
  • Exercise
  • Fiber type specificity
  • L-type voltage-gated calcium channel
  • Muscle plasticity

Cite this

Jeftinija, D. M., Qing, B. W., Hebert, S. L., Norris, C. M., Yan, Z., Rich, M. M., & Kraner, S. D. (2007). The Cav 1.2 Ca2+ channel is expressed in sarcolemma of type I and IIA myofibers of adult skeletal muscle. Muscle and Nerve, 36(4), 482-490. https://doi.org/10.1002/mus.20842

The Cav 1.2 Ca2+ channel is expressed in sarcolemma of type I and IIA myofibers of adult skeletal muscle. / Jeftinija, Dušan M.; Qing, Bo Wang; Hebert, Sadie L.; Norris, Christopher M.; Yan, Zhen; Rich, Mark M.; Kraner, Susan D.

In: Muscle and Nerve, Vol. 36, No. 4, 01.10.2007, p. 482-490.

Research output: Contribution to journalArticle

Jeftinija, Dušan M. ; Qing, Bo Wang ; Hebert, Sadie L. ; Norris, Christopher M. ; Yan, Zhen ; Rich, Mark M. ; Kraner, Susan D. / The Cav 1.2 Ca2+ channel is expressed in sarcolemma of type I and IIA myofibers of adult skeletal muscle. In: Muscle and Nerve. 2007 ; Vol. 36, No. 4. pp. 482-490.
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