The C. Elegans hypodermis couples progenitor cell quiescence to the dietary state

Masamitsu Fukuyama, Kenji Kontani, Toshiaki Katada, Ann E. Rougvie

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The nutritional status of an organism can greatly impact the function and behavior of stem and progenitor cells [1]. However, the regulatory circuits that inform these cells about the dietary environment remain to be elucidated. Newly hatched C. Elegans larvae (L1s) halt development in "L1 arrest" or "L1 diapause" until ample food is encountered and triggers stem and progenitor cells to exit from quiescence [2]. The insulin/insulin-like growth factor signaling (IIS) pathway plays a key role in this reactivation [3, 4], but its site(s) of action have not been elucidated nor have the nutrient molecule(s) that stimulate the pathway been identified. By tissue-specifically modulating the activity of its components, we demonstrate that the IIS pathway acts in the hypodermis to regulate nutrition-responsive reactivation of neural and mesodermal progenitor cells. We identify ethanol, a likely component of the natural Caenorhabditis habitat, and amino acids as nutrients that synergistically reactivate somatic progenitor cells and upregulate expression of insulin-like genes in starved L1 larvae. The hypodermis likely senses the availability of amino acids because forced activation of the amino-acid-responsive Rag-TORC1 (target of rapamycin complex 1) pathway in this tissue can also release somatic progenitor cell quiescence in the presence of ethanol. Finally, there appears to be crosstalk between the IIS and Rag-TORC1 pathways because constitutive activation of the IIS pathway requires Rag to promote reactivation. This work demonstrates that ethanol and amino acids act as dietary cues via the IIS and Rag-TORC1 pathways in the hypodermis to coordinately control progenitor cell behavior.

Original languageEnglish (US)
Pages (from-to)1241-1248
Number of pages8
JournalCurrent Biology
Volume25
Issue number9
DOIs
StatePublished - May 4 2015

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Subcutaneous Tissue
stem cells
Stem Cells
Amino Acids
Ethanol
Nutrients
Chemical activation
Insulin
Tissue
amino acids
ethanol
Somatomedins
Nutrition
Crosstalk
somatic cells
Food
Larva
insulin
Caenorhabditis
Genes

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The C. Elegans hypodermis couples progenitor cell quiescence to the dietary state. / Fukuyama, Masamitsu; Kontani, Kenji; Katada, Toshiaki; Rougvie, Ann E.

In: Current Biology, Vol. 25, No. 9, 04.05.2015, p. 1241-1248.

Research output: Contribution to journalArticle

Fukuyama, Masamitsu ; Kontani, Kenji ; Katada, Toshiaki ; Rougvie, Ann E. / The C. Elegans hypodermis couples progenitor cell quiescence to the dietary state. In: Current Biology. 2015 ; Vol. 25, No. 9. pp. 1241-1248.
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