The Bradyrhizobium japonicum nolA gene and its involvement in the renotype-specific nodulation of soybeans

M. J. Sadowsky, P. B. Cregan, M. Gottfert, A. Sharma, D. Gerhold, F. Rodriguez-Quinones, H. H. Keyser, H. Hennecke, G. Stacey

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91 Scopus citations

Abstract

Several soybean genotypes have been identi which specifically exclude nodulation by members of hizobium japonicum serocluster 123. We have identified d sequenced a DNA region from B. japonicum strain USDA which is involved in genotype-specific nodulation of soyns. This 2.3-kilobase region, cloned in pMJS12, allows B. nicum serocluster 123 isolates to form nodules on plants of group 123-restricting genotypes. The nodules, however, were ineffective for symbiotic nitrogen fixation. The nodulation-complementing region is located approximately 590 base transcriptionally downstream from nodD2. The 5′ end of JS12 contains a putative open reading frame (ORF) of 710 pairs, termed nolA. Transposon Tn3-HoHo mutations y within the ORF abolished nodulation complementation. The N terminus of the predicted nolA gene product has strong rity with the N terminus of MerR, the regulator of cary resistance genes. Translational lacZ fusion experis indicated that nolA was moderately induced by soybean extract and the isoflavone genistein. Restriction fragments hybridize to pMJS12 were detected in genomic DNAs from nodulation-restricted and -unrestricted strains.

Original languageEnglish (US)
Pages (from-to)637-641
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number2
StatePublished - 1991

Keywords

  • Gene-for-gene interactions
  • Nodulation gene
  • Plant-bacteria interaction
  • Symbiosis

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