The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction

Brian D. McCabe; Guillermo Marqués; A. Pejmun Haghighi; Richard D. Fetter; M. Lisa Crotty; Theodore E. Haerry; Corey S. Goodman; Michael B. O'Connor

doi:10.1016/S0896-6273(03)00426-4

The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction

Brian D. McCabe, Guillermo Marqués, A. Pejmun Haghighi, Richard D. Fetter, M. Lisa Crotty, Theodore E. Haerry, Corey S. Goodman, Michael B. O'Connor

Research output: Contribution to journal › Article › peer-review

322 Scopus citations

Abstract

We show that the BMP ortholog Gbb can signal by a retrograde mechanism to regulate synapse growth of the Drosophila neuromuscular junction (NMJ). gbb mutants have a reduced NMJ synapse size, decreased neurotransmitter release, and aberrant presynaptic ultrastructure. These defects are similar to those we observe in mutants of BMP receptors and Smad transcription factors. However, whereas these BMP receptors and signaling components are required in the presynaptic motoneuron, Gbb expression is required in large part in postsynaptic muscles; gbb expression in muscle rescues key aspects of the gbb mutant phenotype. Consistent with this notion, we find that blocking retrograde axonal transport by overexpression of dominant-negative p150/Glued in neurons inhibits BMP signaling in motoneurons. These experiments reveal that a muscle-derived BMP retrograde signal participates in coordinating neuromuscular synapse development and growth.

Original language	English (US)
Pages (from-to)	241-254
Number of pages	14
Journal	Neuron
Volume	39
Issue number	2
DOIs	https://doi.org/10.1016/S0896-6273(03)00426-4
State	Published - Jul 17 2003

Bibliographical note

Funding Information:
We thank Kristy Wharton for generously supplying gbb mutant stocks and UAS-gbb constructs. We are grateful to Grace Panganiban and Peter ten Dijke for sending samples of antibodies. We acknowledge Tom Hays for suggesting the Dynein disruption experiments and we acknowledge Osamu Shimmi for proposing and giving advice in the punt RNAi experiments. We thank the Bloomington Stock center for Drosophila stocks. B.D.M. was supported by a Wellcome Trust Prize Traveling Fellowship. M.B.O. is an associate investigator of the Howard Hughes Medical Institute.

Publisher link

10.1016/S0896-6273(03)00426-4

Find It

Find It at U of M Libraries

Cite this

@article{0678aadfce8140e082c640911d4a183d,

title = "The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction",

abstract = "We show that the BMP ortholog Gbb can signal by a retrograde mechanism to regulate synapse growth of the Drosophila neuromuscular junction (NMJ). gbb mutants have a reduced NMJ synapse size, decreased neurotransmitter release, and aberrant presynaptic ultrastructure. These defects are similar to those we observe in mutants of BMP receptors and Smad transcription factors. However, whereas these BMP receptors and signaling components are required in the presynaptic motoneuron, Gbb expression is required in large part in postsynaptic muscles; gbb expression in muscle rescues key aspects of the gbb mutant phenotype. Consistent with this notion, we find that blocking retrograde axonal transport by overexpression of dominant-negative p150/Glued in neurons inhibits BMP signaling in motoneurons. These experiments reveal that a muscle-derived BMP retrograde signal participates in coordinating neuromuscular synapse development and growth.",

author = "McCabe, {Brian D.} and Guillermo Marqu{\'e}s and Haghighi, {A. Pejmun} and Fetter, {Richard D.} and Crotty, {M. Lisa} and Haerry, {Theodore E.} and Goodman, {Corey S.} and O'Connor, {Michael B.}",

note = "Funding Information: We thank Kristy Wharton for generously supplying gbb mutant stocks and UAS-gbb constructs. We are grateful to Grace Panganiban and Peter ten Dijke for sending samples of antibodies. We acknowledge Tom Hays for suggesting the Dynein disruption experiments and we acknowledge Osamu Shimmi for proposing and giving advice in the punt RNAi experiments. We thank the Bloomington Stock center for Drosophila stocks. B.D.M. was supported by a Wellcome Trust Prize Traveling Fellowship. M.B.O. is an associate investigator of the Howard Hughes Medical Institute.",

year = "2003",

month = jul,

day = "17",

doi = "10.1016/S0896-6273(03)00426-4",

language = "English (US)",

volume = "39",

pages = "241--254",

journal = "Neuron",

issn = "0896-6273",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction

AU - McCabe, Brian D.

AU - Marqués, Guillermo

AU - Haghighi, A. Pejmun

AU - Fetter, Richard D.

AU - Crotty, M. Lisa

AU - Haerry, Theodore E.

AU - Goodman, Corey S.

AU - O'Connor, Michael B.

N1 - Funding Information: We thank Kristy Wharton for generously supplying gbb mutant stocks and UAS-gbb constructs. We are grateful to Grace Panganiban and Peter ten Dijke for sending samples of antibodies. We acknowledge Tom Hays for suggesting the Dynein disruption experiments and we acknowledge Osamu Shimmi for proposing and giving advice in the punt RNAi experiments. We thank the Bloomington Stock center for Drosophila stocks. B.D.M. was supported by a Wellcome Trust Prize Traveling Fellowship. M.B.O. is an associate investigator of the Howard Hughes Medical Institute.

PY - 2003/7/17

Y1 - 2003/7/17

N2 - We show that the BMP ortholog Gbb can signal by a retrograde mechanism to regulate synapse growth of the Drosophila neuromuscular junction (NMJ). gbb mutants have a reduced NMJ synapse size, decreased neurotransmitter release, and aberrant presynaptic ultrastructure. These defects are similar to those we observe in mutants of BMP receptors and Smad transcription factors. However, whereas these BMP receptors and signaling components are required in the presynaptic motoneuron, Gbb expression is required in large part in postsynaptic muscles; gbb expression in muscle rescues key aspects of the gbb mutant phenotype. Consistent with this notion, we find that blocking retrograde axonal transport by overexpression of dominant-negative p150/Glued in neurons inhibits BMP signaling in motoneurons. These experiments reveal that a muscle-derived BMP retrograde signal participates in coordinating neuromuscular synapse development and growth.

AB - We show that the BMP ortholog Gbb can signal by a retrograde mechanism to regulate synapse growth of the Drosophila neuromuscular junction (NMJ). gbb mutants have a reduced NMJ synapse size, decreased neurotransmitter release, and aberrant presynaptic ultrastructure. These defects are similar to those we observe in mutants of BMP receptors and Smad transcription factors. However, whereas these BMP receptors and signaling components are required in the presynaptic motoneuron, Gbb expression is required in large part in postsynaptic muscles; gbb expression in muscle rescues key aspects of the gbb mutant phenotype. Consistent with this notion, we find that blocking retrograde axonal transport by overexpression of dominant-negative p150/Glued in neurons inhibits BMP signaling in motoneurons. These experiments reveal that a muscle-derived BMP retrograde signal participates in coordinating neuromuscular synapse development and growth.

UR - http://www.scopus.com/inward/record.url?scp=0042964820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042964820&partnerID=8YFLogxK

U2 - 10.1016/S0896-6273(03)00426-4

DO - 10.1016/S0896-6273(03)00426-4

M3 - Article

C2 - 12873382

AN - SCOPUS:0042964820

SN - 0896-6273

VL - 39

SP - 241

EP - 254

JO - Neuron

JF - Neuron

IS - 2

ER -

The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

University Imaging Centers

Cite this

The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

University Assets

University Imaging Centers

Cite this