The bacterial stressosome: A modular system that has been adapted to control secondary messenger signaling

Maureen B. Quin; John M. Berrisford; Joseph A. Newman; Arnaud Baslé; Richard J. Lewis; Jon Marles-Wright

doi:10.1016/j.str.2012.01.003

The bacterial stressosome: A modular system that has been adapted to control secondary messenger signaling

Maureen B. Quin, John M. Berrisford, Joseph A. Newman, Arnaud Baslé, Richard J. Lewis, Jon Marles-Wright

Biotechnology Institute

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The stressosome complex regulates downstream effectors in response to environmental signals. In Bacillus subtilis, it activates the alternative sigma factor σ ^B, leading to the upregulation of the general stress regulon. Herein, we characterize a stressosome-regulated biochemical pathway in Moorella thermoacetica. We show that the presumed sensor, MtR, and the scaffold, MtS, form a pseudo-icosahedral structure like that observed in B. subtilis. The N-terminal domain of MtR is structurally homologous to B. subtilis RsbR, despite low sequence identity. The affinity of the switch kinase, MtT, for MtS decreases following MtS phosphorylation and not because of structural reorganization. Dephosphorylation of MtS by the PP2C type phosphatase MtX permits the switch kinase to rebind the stressosome to reset the response. We also show that MtT regulates cyclic di-GMP biosynthesis through inhibition of a GG(D/E)EF-type diguanylate cyclase, demonstrating that secondary messenger levels are regulated by the stressosome.

Original language	English (US)
Pages (from-to)	350-363
Number of pages	14
Journal	Structure
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/j.str.2012.01.003
State	Published - Feb 8 2012

Bibliographical note

Funding Information:
This work was supported by grants from the BBSRC (BBG001553 and BBF001533). We would like to thank Dr. S. Ragsdale for the kind gift of M. thermoacetica genomic DNA and Dr. T. Schirmer for the kind gift of an expression plasmid containing E. coli ydeH . We thank the staff and beamline scientists at Diamond Light Source and the ESRF for their help with data collection and members of the Lewis laboratory for critical reading of the manuscript.

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abstract = "The stressosome complex regulates downstream effectors in response to environmental signals. In Bacillus subtilis, it activates the alternative sigma factor σ B, leading to the upregulation of the general stress regulon. Herein, we characterize a stressosome-regulated biochemical pathway in Moorella thermoacetica. We show that the presumed sensor, MtR, and the scaffold, MtS, form a pseudo-icosahedral structure like that observed in B. subtilis. The N-terminal domain of MtR is structurally homologous to B. subtilis RsbR, despite low sequence identity. The affinity of the switch kinase, MtT, for MtS decreases following MtS phosphorylation and not because of structural reorganization. Dephosphorylation of MtS by the PP2C type phosphatase MtX permits the switch kinase to rebind the stressosome to reset the response. We also show that MtT regulates cyclic di-GMP biosynthesis through inhibition of a GG(D/E)EF-type diguanylate cyclase, demonstrating that secondary messenger levels are regulated by the stressosome.",

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note = "Funding Information: This work was supported by grants from the BBSRC (BBG001553 and BBF001533). We would like to thank Dr. S. Ragsdale for the kind gift of M. thermoacetica genomic DNA and Dr. T. Schirmer for the kind gift of an expression plasmid containing E. coli ydeH . We thank the staff and beamline scientists at Diamond Light Source and the ESRF for their help with data collection and members of the Lewis laboratory for critical reading of the manuscript. ",

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AU - Lewis, Richard J.

AU - Marles-Wright, Jon

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N2 - The stressosome complex regulates downstream effectors in response to environmental signals. In Bacillus subtilis, it activates the alternative sigma factor σ B, leading to the upregulation of the general stress regulon. Herein, we characterize a stressosome-regulated biochemical pathway in Moorella thermoacetica. We show that the presumed sensor, MtR, and the scaffold, MtS, form a pseudo-icosahedral structure like that observed in B. subtilis. The N-terminal domain of MtR is structurally homologous to B. subtilis RsbR, despite low sequence identity. The affinity of the switch kinase, MtT, for MtS decreases following MtS phosphorylation and not because of structural reorganization. Dephosphorylation of MtS by the PP2C type phosphatase MtX permits the switch kinase to rebind the stressosome to reset the response. We also show that MtT regulates cyclic di-GMP biosynthesis through inhibition of a GG(D/E)EF-type diguanylate cyclase, demonstrating that secondary messenger levels are regulated by the stressosome.

AB - The stressosome complex regulates downstream effectors in response to environmental signals. In Bacillus subtilis, it activates the alternative sigma factor σ B, leading to the upregulation of the general stress regulon. Herein, we characterize a stressosome-regulated biochemical pathway in Moorella thermoacetica. We show that the presumed sensor, MtR, and the scaffold, MtS, form a pseudo-icosahedral structure like that observed in B. subtilis. The N-terminal domain of MtR is structurally homologous to B. subtilis RsbR, despite low sequence identity. The affinity of the switch kinase, MtT, for MtS decreases following MtS phosphorylation and not because of structural reorganization. Dephosphorylation of MtS by the PP2C type phosphatase MtX permits the switch kinase to rebind the stressosome to reset the response. We also show that MtT regulates cyclic di-GMP biosynthesis through inhibition of a GG(D/E)EF-type diguanylate cyclase, demonstrating that secondary messenger levels are regulated by the stressosome.

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The bacterial stressosome: A modular system that has been adapted to control secondary messenger signaling

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