TY - JOUR
T1 - The atzABC genes encoding atrazine catabolism are located on a self- transmissible plasmid in Pseudomonas sp. strain ADP
AU - De Souza, Mervyn L.
AU - Wackett, Lawrence P.
AU - Sadowsky, Michael J.
PY - 1998/6
Y1 - 1998/6
N2 - Pseudomonas sp. strain ADP initiates atrazine catabolism via three enzymatic steps, encoded by atzA, -B, and -C, which yield cyanuric acid, a nitrogen source for many bacteria. In-well lysis, Southern hybridization, and plasmid transfer studies indicated that the atzA, -B, and -C genes are localized on a 96-kb self-transmissible plasmid, pADP-I, in Pseudomonas sp. strain ADP. High-performance liquid chromatography analyses showed that cyanuric acid degradation was not encoded by pADP-I. pADP-1 was transferred to Escherichia coli strains at a frequency of 4.7 x 10-2. This suggests a potential molecular mechanism for the dispersion of the atzABC genes to other soil bacteria.
AB - Pseudomonas sp. strain ADP initiates atrazine catabolism via three enzymatic steps, encoded by atzA, -B, and -C, which yield cyanuric acid, a nitrogen source for many bacteria. In-well lysis, Southern hybridization, and plasmid transfer studies indicated that the atzA, -B, and -C genes are localized on a 96-kb self-transmissible plasmid, pADP-I, in Pseudomonas sp. strain ADP. High-performance liquid chromatography analyses showed that cyanuric acid degradation was not encoded by pADP-I. pADP-1 was transferred to Escherichia coli strains at a frequency of 4.7 x 10-2. This suggests a potential molecular mechanism for the dispersion of the atzABC genes to other soil bacteria.
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U2 - 10.1128/aem.64.6.2323-2326.1998
DO - 10.1128/aem.64.6.2323-2326.1998
M3 - Article
C2 - 9603862
AN - SCOPUS:0031745940
SN - 0099-2240
VL - 64
SP - 2323
EP - 2326
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 6
ER -