The atzABC genes encoding atrazine catabolism are located on a self- transmissible plasmid in Pseudomonas sp. strain ADP

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Abstract

Pseudomonas sp. strain ADP initiates atrazine catabolism via three enzymatic steps, encoded by atzA, -B, and -C, which yield cyanuric acid, a nitrogen source for many bacteria. In-well lysis, Southern hybridization, and plasmid transfer studies indicated that the atzA, -B, and -C genes are localized on a 96-kb self-transmissible plasmid, pADP-I, in Pseudomonas sp. strain ADP. High-performance liquid chromatography analyses showed that cyanuric acid degradation was not encoded by pADP-I. pADP-1 was transferred to Escherichia coli strains at a frequency of 4.7 x 10-2. This suggests a potential molecular mechanism for the dispersion of the atzABC genes to other soil bacteria.

Original languageEnglish (US)
Pages (from-to)2323-2326
Number of pages4
JournalApplied and environmental microbiology
Volume64
Issue number6
DOIs
StatePublished - Jun 1998

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