Purpose: To explore the association between consumption of fruits and vegetables and the presence of glaucoma in older African-American women. Design: Cross-sectional study. Methods: Disc photographs and suprathreshold visual fields were obtained from the 662 African-American participants in the Study of Osteoporotic Fractures. Masked, trained readers graded all discs, and 2 glaucoma specialists reviewed photographs and visual fields. The Block Food Frequency Questionnaire assessed food consumption. Relationships between selected fruit/vegetable/nutrient consumption and glaucoma were evaluated using logistic regression models after adjusting for potential confounders. Results: After excluding women missing Food Frequency Questionnaire and disc data, 584 African-American women (88.2% of total African-American cohort) were included. Glaucoma was diagnosed in at least 1 eye in 77 subjects (13%). Women who ate 3 or more servings/day of fruits/fruit juices were 79% (odds ratio [OR] = 0.21; 95% confidence interval [CI]: 0.08-0.60) less likely to have glaucoma than women who ate less than 1 serving/day. Women who consumed more than 2 servings/week of fresh oranges (OR = 0.18; 95% CI: 0.06-0.51) and peaches (OR = 0.30; 95% CI: 0.13-0.67) had a decreased odds of glaucoma compared to those consuming less than 1 serving/week. For vegetables, >1 serving/week compared to ≤1 serving/month of collard greens/kale decreased the odds of glaucoma by 57% (OR = 0.43; 95% CI: 0.21-0.85). There was a protective trend against glaucoma in those consuming more fruit/fruit juices (P =.023), fresh oranges (P =.002), fresh peaches (P =.002), and collard greens/kale (P =.014). Higher consumption of carrots (P =.061) and spinach (P =.094) also showed some associations. Individual nutrient intake from food sources found protective trends with higher intakes of vitamin A (P =.011), vitamin C (P =.018), and α-carotene (P =.021), and close to statistically significant trends with β-carotene (P =.052), folate (P =.056), and lutein/zeaxanthin (P =.077). Conclusion: Higher intake of certain fruits and vegetables high in vitamins A and C and carotenoids may be associated with a decreased likelihood of glaucoma in older African-American women. Randomized controlled trials are needed to determine whether the intake of specific nutrients changes the risk of glaucoma.