The assembly of functional preinitiation complexes and transcription of 5S RNA-encoding genes containing point mutations

Barkur S. Shastry, David Greenstein

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The transcription of several Syrian hamster 5S RNA-encoding genes (5S genes) containing single and multiple point mutations in and around the intragenic control region has been analyzed in a HeLa cell-free system. Although most genes with point mutations displayed normal levels of transcription, several exhibited a three- to fivefold reduction in transcription. These mutations interfere with the interaction between the 5S genes and the soluble factors. The above studies help to establish the importance of specific nucleotides within the 5S gene for productive interactions of individual transcription factors in vitro.

Original languageEnglish (US)
Pages (from-to)269-278
Number of pages10
JournalGene
Volume107
Issue number2
DOIs
StatePublished - Nov 15 1991

Keywords

  • (Hamster
  • RNA polymerase III
  • footprinting
  • mutations
  • recombinant DNA
  • stable complex)

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