The Architecture of Inactivated SARS-CoV-2 with Postfusion Spikes Revealed by Cryo-EM and Cryo-ET

Chuang Liu, Luiza Mendonça, Yang Yang, Yuanzhu Gao, Chenguang Shen, Jiwei Liu, Tao Ni, Bin Ju, Congcong Liu, Xian Tang, Jinli Wei, Xiaomin Ma, Yanan Zhu, Weilong Liu, Shuman Xu, Yingxia Liu, Jing Yuan, Jing Wu, Zheng Liu, Zheng ZhangLei Liu, Peiyi Wang, Peijun Zhang

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

Several vaccine candidates using inactivated SARS-CoV-2 viruses are under development. Liu et al. used state-of-the-art cryoelectron microscopy technologies to characterize the architecture of inactivated SARS-CoV-2 viruses. They found that the viral spikes are mostly in a postfusion state, which is not desirable for vaccine development.

Original languageEnglish (US)
Pages (from-to)1218-1224.e4
JournalStructure
Volume28
Issue number11
DOIs
StatePublished - Nov 3 2020
Externally publishedYes

Bibliographical note

Funding Information:
This research was supported by the Science and Technology Innovation Committee of Shenzhen Municipality ( 202002073000002 and JSGG 20200207155251653 ) and National Institutes of Health grant P50AI150481 (P.Z.), UK Wellcome Trust Investigator Award 206422/Z/17/Z (P.Z.), and UK Biotechnology and Biological Sciences Research Council grant BB/S003339/1 (P.Z.).

Publisher Copyright:
© 2020 The Authors

Keywords

  • COVID-19
  • cryo-EM
  • cryo-ET
  • glycosylation
  • postfusion
  • SARS-CoV-2
  • spike
  • subtomogram averaging
  • vaccine
  • β-propiolactone-inactivated viruses

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