The Arabidopsis auxin receptor F-box proteins AFB4 and AFB5 are required for response to the synthetic auxin picloram

Michael J. Prigge, Kathleen Greenham, Yi Zhang, Aaron Santner, Cristina Castillejo, Andrew M. Mutka, Ronan C. O'Malley, Joseph R. Ecker, Barbara N. Kunkel, Mark Estelle

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The plant hormone auxin is perceived by a family of F-box proteins called the TIR1/AFBs. Phylogenetic studies reveal that these proteins fall into four clades in flowering plants called TIR1, AFB2, AFB4, and AFB6. Genetic studies indicate that members of the TIR1 and AFB2 groups act as positive regulators of auxin signaling by promoting the degradation of the Aux/IAA transcriptional repressors. In this report, we demonstrate that both AFB4 and AFB5 also function as auxin receptors based on in vitro assays. We also provide genetic evidence that AFB4 and AFB5 are targets of the picloram family of auxinic herbicides in addition to indole-3-acetic acid. In contrast to previous studies we find that null afb4 alleles do not exhibit obvious defects in seedling morphology or auxin hypersensitivity. We conclude that AFB4 and AFB5 act in a similar fashion to other members of the family but exhibit a distinct auxin specificity.

Original languageEnglish (US)
Pages (from-to)1383-1390
Number of pages8
JournalG3: Genes, Genomes, Genetics
Volume6
Issue number5
DOIs
StatePublished - May 1 2016
Externally publishedYes

Bibliographical note

Funding Information:
We thank Nikita Kadakia and Vanessa Peterson for technical assistance. Work in the authors' lab was supported by a grant from the National Institutes of Health (GM43644 to M.E.) and from the National Science Foundation (MCB-1122250 to J.R.E.).

Keywords

  • Arabidopsis
  • Auxin
  • F-box protein

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