The antimicrobial peptide DGL13K is active against drug-resistant gram-negative bacteria and sub-inhibitory concentrations stimulate bacterial growth without causing resistance

Sven Ulrik Gorr, Hunter V. Brigman, Jadyn C. Anderson, Elizabeth B. Hirsch

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Antimicrobial peptides may be alternatives to traditional antibiotics with reduced bacterial resistance. The antimicrobial peptide GL13K was derived from the salivary protein BPIFA2. This study determined the relative activity of the L-and D-enantiomers of GL13K to wild-type and drug-resistant strains of three gram-negative species and against Pseudomonas aeruginosa biofilms. DGL13K displayed in vitro activity against extended-spectrum beta-lactamase (ESBL)-producing and Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (MICs 16-32 μg/ml), MDR and XDR P. aeruginosa, and XDR Acinetobacter baumannii carrying metallo-beta-lactamases (MICs 8-32 μg/ml). P. aeruginosa showed low inherent resistance to DGL13K and the increased metabolic activity and growth caused by sub-MIC concentrations of GL13K peptides did not result in acquired bacterial resistance. Daily treatment for approximately two weeks did not increase the MIC of DGL13K or cause cross-resistance between LGL13K and DGL13K. These data suggest that DGL13K is a promising antimicrobial peptide candidate for further development.

Original languageEnglish (US)
Article numbere0273504
JournalPloS one
Volume17
Issue number8 August
DOIs
StatePublished - Aug 2022

Bibliographical note

Publisher Copyright:
© 2022 Gorr et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Keywords

  • Anti-Bacterial Agents/pharmacology
  • Antimicrobial Peptides
  • Drug Resistance, Multiple, Bacterial
  • Gram-Negative Bacteria/metabolism
  • Klebsiella pneumoniae/metabolism
  • Microbial Sensitivity Tests
  • Pseudomonas aeruginosa/metabolism
  • beta-Lactamases/metabolism

PubMed: MeSH publication types

  • Journal Article

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