The affinity of allergen specific IgE and the competition between IgE and IgG for the allergen in Amb a V sensitive individuals

Kyu Earn Kim, Andreas Rosenberg, Sean Roberts, Malcolm N Blumenthal

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We have calibrated a solid state PAST assay with affinity purified allergen-specific IgE. We then utilized the calibrated assay to measure the average affinity of individual IgE-containing sera in terms of the average association constant (K) for purified allergen Amb a V. The binding data yielded linear reciprocal plots indicating that the range of affinities of the responding crones was narrow. The range of the average association constant for the IgE-Amb a V complex was 0.9-26 x 1010 M-1. The average affinity of the corresponding IgG response in the same individual, estimated by inhibition studies of IgE binding, was 107 M-1 in one case and lower than 106 M-1 in all the other cases.

Original languageEnglish (US)
Pages (from-to)873-880
Number of pages8
JournalMolecular Immunology
Volume33
Issue number10
DOIs
StatePublished - Jul 1996

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