Abstract
Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a Km for acetate of 160 mM, and a kcat of 16,800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 418-420 |
| Number of pages | 3 |
| Journal | Archives of Microbiology |
| Volume | 166 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1996 |
Keywords
- Acetate kinase
- Acetate utilization
- Clostridum acetobutylicum
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