The acetate kinase of Clostridum acetobutylicum strain P262

Francisco Diez-Gonzalez; James B. Russell; Jean B. Hunter

The acetate kinase of Clostridum acetobutylicum strain P262

Francisco Diez-Gonzalez, James B. Russell, Jean B. Hunter

Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a K_m for acetate of 160 mM, and a k_cat of 16,800 min^-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.

Original language	English (US)
Pages (from-to)	418-420
Number of pages	3
Journal	Archives of Microbiology
Volume	166
Issue number	6
State	Published - Dec 1 1996

Keywords

Acetate kinase
Acetate utilization
Clostridum acetobutylicum

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title = "The acetate kinase of Clostridum acetobutylicum strain P262",

abstract = "Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a Km for acetate of 160 mM, and a kcat of 16,800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.",

keywords = "Acetate kinase, Acetate utilization, Clostridum acetobutylicum",

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T1 - The acetate kinase of Clostridum acetobutylicum strain P262

AU - Diez-Gonzalez, Francisco

AU - Russell, James B.

AU - Hunter, Jean B.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a Km for acetate of 160 mM, and a kcat of 16,800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.

AB - Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a Km for acetate of 160 mM, and a kcat of 16,800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.

KW - Acetate kinase

KW - Acetate utilization

KW - Clostridum acetobutylicum

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