TY - JOUR
T1 - The acetate kinase of Clostridum acetobutylicum strain P262
AU - Diez-Gonzalez, Francisco
AU - Russell, James B.
AU - Hunter, Jean B.
PY - 1996/12/1
Y1 - 1996/12/1
N2 - Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a Km for acetate of 160 mM, and a kcat of 16,800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.
AB - Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a Km for acetate of 160 mM, and a kcat of 16,800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.
KW - Acetate kinase
KW - Acetate utilization
KW - Clostridum acetobutylicum
UR - http://www.scopus.com/inward/record.url?scp=0030299889&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030299889&partnerID=8YFLogxK
M3 - Article
C2 - 9082921
AN - SCOPUS:0030299889
VL - 166
SP - 418
EP - 420
JO - Archives of Microbiology
JF - Archives of Microbiology
SN - 0302-8933
IS - 6
ER -