Thatcher wheat line RL6149 carries Lr64 and a second leaf rust resistance gene on chromosome 1DS

J. A. Kolmer, A. Bernardo, G. Bai, M. J. Hayden, J. A. Anderson

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


KEY MESSAGE: The leaf rust resistance gene Lr64 in the Thatcher wheat RL6149 was mapped to chromosome 6AL with SNP and KASP markers and a second leaf rust resistance gene was mapped to chromosome 1DS. RL6149, a near-isogenic line of Thatcher wheat, carries leaf rust resistance gene Lr64 on chromosome arm 6AL. The objective of this study was to develop molecular markers that can be easily used to select wheat lines with Lr64. RL6149 was crossed with Thatcher and F 2 plants derived from a single F 1 plant were advanced to F 6 lines by single seed descent. The 100 F 7 recombinant inbred lines (RIL) were inoculated with two races of P.triticina that differed widely for virulence in order to identify resistant and susceptible RIL. Thirty RIL that differed for resistance and the parental lines were genotyped with the 90 K Infinium iSelect single nucleotide polymorphism (SNP) array to find closely linked markers with Lr64. Seven linked SNPs on chromosome arm 6AL were converted into Kompetitive Allele Specific PCR (KASP) markers that were genotyped on the 100 RIL. A genetic linkage map for the seven KASP markers spanned 19.1 cM on chromosome arm 6AL. KASP marker K-IWB59855 was tightly linked to Lr64. A second unexpected gene for leaf rust resistance also segregated in the F 7 lines. Four KASP markers that spanned 18.6 cM located the gene on chromosome 1DS. The KASP marker K-IWB38437 was tightly linked to the second leaf rust resistance gene.

Original languageEnglish (US)
Pages (from-to)2809-2814
Number of pages6
JournalTheoretical and Applied Genetics
Issue number10
StatePublished - Oct 1 2019

Bibliographical note

Publisher Copyright:
© 2019, This is a U.S. government work and its text is not subject to copyright protection in the United States; however, its text may be subject to foreign copyright protection.


  • Brown rust
  • Race-specific resistance
  • Seedling resistance gene

PubMed: MeSH publication types

  • Journal Article


Dive into the research topics of 'Thatcher wheat line RL6149 carries Lr64 and a second leaf rust resistance gene on chromosome 1DS'. Together they form a unique fingerprint.

Cite this