TGF-β2 secretion from RPE decreases with polarization and becomes apically oriented

Louis Hirsch; Hossein Nazari; Parameswaran G. Sreekumar; Ram Kannan; Laurie Dustin; Danhong Zhu; Ernesto Barron; David R. Hinton

doi:10.1016/j.cyto.2014.11.014

TGF-β2 secretion from RPE decreases with polarization and becomes apically oriented

Louis Hirsch, Hossein Nazari, Parameswaran G. Sreekumar, Ram Kannan, Laurie Dustin, Danhong Zhu, Ernesto Barron, David R. Hinton

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Retinal pigmented epithelium (RPE) secretes transforming growth factor beta 1 and 2 (TGF-β1 and -β2) cytokines involved in fibrosis, immune privilege, and proliferative vitreoretinopathy (PVR). Since RPE cell polarity may be altered in various disease conditions including PVR and age-related macular degeneration, we determined levels of TGF-β from polarized human RPE (hRPE) and human stem cell derived RPE (hESC-RPE) as compared to nonpolarized cells. TGF-β2 was the predominant isoform in all cell culture conditions. Nonpolarized cells secreted significantly more TGF-β2 supporting the contention that loss of polarity of RPE in PVR leads to rise of intravitreal TGF-β2. Active TGF-β2, secreted mainly from apical side of polarized RPE, represented 6-10% of total TGF-β2. In conclusion, polarity is an important determinant of TGF-β2 secretion in RPE. Low levels of apically secreted active TGF-β2 may play a role in the normal physiology of the subretinal space. Comparable secretion of TGF-β from polarized hESC-RPE and hRPE supports the potential for hESC-RPE in RPE replacement therapies.

Original language	English (US)
Pages (from-to)	394-396
Number of pages	3
Journal	Cytokine
Volume	71
Issue number	2
DOIs	https://doi.org/10.1016/j.cyto.2014.11.014
State	Published - Feb 1 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014 Elsevier Ltd.

Keywords

Embryonic stem cell
Polarity
Proliferative vitreoretinopathy
Retinal pigment epithelium
TGF-β

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cyto.2014.11.014

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title = "TGF-β2 secretion from RPE decreases with polarization and becomes apically oriented",

abstract = "Retinal pigmented epithelium (RPE) secretes transforming growth factor beta 1 and 2 (TGF-β1 and -β2) cytokines involved in fibrosis, immune privilege, and proliferative vitreoretinopathy (PVR). Since RPE cell polarity may be altered in various disease conditions including PVR and age-related macular degeneration, we determined levels of TGF-β from polarized human RPE (hRPE) and human stem cell derived RPE (hESC-RPE) as compared to nonpolarized cells. TGF-β2 was the predominant isoform in all cell culture conditions. Nonpolarized cells secreted significantly more TGF-β2 supporting the contention that loss of polarity of RPE in PVR leads to rise of intravitreal TGF-β2. Active TGF-β2, secreted mainly from apical side of polarized RPE, represented 6-10% of total TGF-β2. In conclusion, polarity is an important determinant of TGF-β2 secretion in RPE. Low levels of apically secreted active TGF-β2 may play a role in the normal physiology of the subretinal space. Comparable secretion of TGF-β from polarized hESC-RPE and hRPE supports the potential for hESC-RPE in RPE replacement therapies.",

keywords = "Embryonic stem cell, Polarity, Proliferative vitreoretinopathy, Retinal pigment epithelium, TGF-β",

author = "Louis Hirsch and Hossein Nazari and Sreekumar, {Parameswaran G.} and Ram Kannan and Laurie Dustin and Danhong Zhu and Ernesto Barron and Hinton, {David R.}",

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doi = "10.1016/j.cyto.2014.11.014",

language = "English (US)",

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T1 - TGF-β2 secretion from RPE decreases with polarization and becomes apically oriented

AU - Hirsch, Louis

AU - Nazari, Hossein

AU - Sreekumar, Parameswaran G.

AU - Kannan, Ram

AU - Dustin, Laurie

AU - Zhu, Danhong

AU - Barron, Ernesto

AU - Hinton, David R.

PY - 2015/2/1

Y1 - 2015/2/1

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AB - Retinal pigmented epithelium (RPE) secretes transforming growth factor beta 1 and 2 (TGF-β1 and -β2) cytokines involved in fibrosis, immune privilege, and proliferative vitreoretinopathy (PVR). Since RPE cell polarity may be altered in various disease conditions including PVR and age-related macular degeneration, we determined levels of TGF-β from polarized human RPE (hRPE) and human stem cell derived RPE (hESC-RPE) as compared to nonpolarized cells. TGF-β2 was the predominant isoform in all cell culture conditions. Nonpolarized cells secreted significantly more TGF-β2 supporting the contention that loss of polarity of RPE in PVR leads to rise of intravitreal TGF-β2. Active TGF-β2, secreted mainly from apical side of polarized RPE, represented 6-10% of total TGF-β2. In conclusion, polarity is an important determinant of TGF-β2 secretion in RPE. Low levels of apically secreted active TGF-β2 may play a role in the normal physiology of the subretinal space. Comparable secretion of TGF-β from polarized hESC-RPE and hRPE supports the potential for hESC-RPE in RPE replacement therapies.

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KW - Retinal pigment epithelium

KW - TGF-β

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TGF-β2 secretion from RPE decreases with polarization and becomes apically oriented

Abstract

Bibliographical note

Keywords

UN SDGs

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