Textural performance of crosslinked or reduced-calcium milk protein ingredients in model high-protein nutrition bars

J. C. Banach, S. Clark, L. E. Metzger, B. P. Lamsal

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Transglutaminase (Tgase) crosslinking and calcium reduction were investigated as ways to improve the texture and storage stability of high-protein nutrition (HPN) bars formulated with milk protein concentrate (MPC) and micellar casein concentrate (MCC). The MPC and MCC crosslinked at none, low, and high levels, and a reduced-calcium MPC (RCMPC) were each formulated into model HPN bars. Hardness, crumbliness, moisture content, pH, color, and water activity of the HPN bars were measured during accelerated storage. The HPN bars prepared with MPC were harder and more cohesive than those prepared with MCC. Higher levels of Tgase crosslinking improved HPN bar cohesiveness and decreased hardening during storage. The RCMPC produced softer, yet crumblier HPN bars. Small textural differences were observed for the HPN bars formulated with the transglutaminase crosslinked proteins or RCMPC when compared with their respective controls. However, modification only slightly improved protein ingredient ability to slow hardening while balancing cohesion and likely requires further improvement for increased applicability in soft-texture HPN bars.

Original languageEnglish (US)
Pages (from-to)6061-6070
Number of pages10
JournalJournal of Dairy Science
Issue number8
StatePublished - Aug 1 2016

Bibliographical note

Funding Information:
The protein ingredients used in this study were produced at South Dakota State University by Prafulla Salunke and Chenchaiah Marella. This project was partially supported by Dairy Research Institute award #H003889501 through the University of Minnesota and partially by the Iowa State University Agricultural Experiment Station.

Publisher Copyright:
© 2016 American Dairy Science Association


  • micellar casein concentrate
  • milk protein concentrate
  • protein bar
  • transglutaminase


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