Tethering peptides onto biomimetic and injectable nanofiber microspheres to direct cellular response

Johnson V. John; Meera Choksi; Shixuan Chen; Sunil Kumar Boda; Yajuan Su; Alec McCarthy; Matthew J. Teusink; Richard A. Reinhardt; Jingwei Xie

doi:10.1016/j.nano.2019.102081

Tethering peptides onto biomimetic and injectable nanofiber microspheres to direct cellular response

Johnson V. John, Meera Choksi, Shixuan Chen, Sunil Kumar Boda, Yajuan Su, Alec McCarthy, Matthew J. Teusink, Richard A. Reinhardt, Jingwei Xie

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Biomimetic and injectable nanofiber microspheres (NMs) could be ideal candidate for minimally invasive tissue repair. Herein, we report a facile approach to fabricate peptide-tethered NMs by combining electrospinning, electrospraying, and surface conjugation techniques. The composition and size of NMs can be tuned by varying the processing parameters during the fabrication. Further, bone morphogenic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) mimicking peptides have been successfully tethered onto poly(ε-caprolactone) (PCL):gelatin:(gelatin-methacryloyl) (GelMA)(1:0.5:0.5) NMs through photocrosslinking of the methacrylic group in GelMA and octenyl alanine (OCTAL) in the modified peptides. The BMP-2-OCTAL peptide-tethered NMs significantly promote osteogenic differentiation of bone marrow-derived stem cells (BMSCs). Moreover, human umbilical vein endothelial cells (HUVECs) seeded on VEGF mimicking peptide QK-OCTAL-tethered NMs significantly up-regulated vascular-specific proteins, leading to microvascularization. The strategy developed in this work holds great potential in developing a biomimetic and injectable carrier to efficiently direct cellular response (Osteogenesis and Angiogenesis) for tissue repair.

Original language	English (US)
Article number	102081
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	22
DOIs	https://doi.org/10.1016/j.nano.2019.102081
State	Published - Nov 2019

Bibliographical note

Funding Information:
Financial Support Information: This work was supported by grants from the National Institute of General Medical Science (NIGMS) at the NIH (2P20 GM103480), National Institute of Dental and Craniofacial Research (NIDCR) at the NIH (1R21DE027516), NE LB606, and startup funds from the University of Nebraska Medical Center. Conflict of Interest: None.

Publisher Copyright:
© 2019 Elsevier Inc.

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

Cellular response
Microvascularization
Nanofiber microspheres
Osteogenic differentiation
Peptides conjugation

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10.1016/j.nano.2019.102081

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title = "Tethering peptides onto biomimetic and injectable nanofiber microspheres to direct cellular response",

abstract = "Biomimetic and injectable nanofiber microspheres (NMs) could be ideal candidate for minimally invasive tissue repair. Herein, we report a facile approach to fabricate peptide-tethered NMs by combining electrospinning, electrospraying, and surface conjugation techniques. The composition and size of NMs can be tuned by varying the processing parameters during the fabrication. Further, bone morphogenic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) mimicking peptides have been successfully tethered onto poly(ε-caprolactone) (PCL):gelatin:(gelatin-methacryloyl) (GelMA)(1:0.5:0.5) NMs through photocrosslinking of the methacrylic group in GelMA and octenyl alanine (OCTAL) in the modified peptides. The BMP-2-OCTAL peptide-tethered NMs significantly promote osteogenic differentiation of bone marrow-derived stem cells (BMSCs). Moreover, human umbilical vein endothelial cells (HUVECs) seeded on VEGF mimicking peptide QK-OCTAL-tethered NMs significantly up-regulated vascular-specific proteins, leading to microvascularization. The strategy developed in this work holds great potential in developing a biomimetic and injectable carrier to efficiently direct cellular response (Osteogenesis and Angiogenesis) for tissue repair.",

keywords = "Cellular response, Microvascularization, Nanofiber microspheres, Osteogenic differentiation, Peptides conjugation",

author = "John, {Johnson V.} and Meera Choksi and Shixuan Chen and Boda, {Sunil Kumar} and Yajuan Su and Alec McCarthy and Teusink, {Matthew J.} and Reinhardt, {Richard A.} and Jingwei Xie",

note = "Funding Information: Financial Support Information: This work was supported by grants from the National Institute of General Medical Science (NIGMS) at the NIH (2P20 GM103480), National Institute of Dental and Craniofacial Research (NIDCR) at the NIH (1R21DE027516), NE LB606, and startup funds from the University of Nebraska Medical Center. Conflict of Interest: None. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

year = "2019",

month = nov,

doi = "10.1016/j.nano.2019.102081",

language = "English (US)",

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journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",

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AU - Choksi, Meera

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AU - Boda, Sunil Kumar

AU - Su, Yajuan

AU - McCarthy, Alec

AU - Teusink, Matthew J.

AU - Reinhardt, Richard A.

AU - Xie, Jingwei

N1 - Funding Information: Financial Support Information: This work was supported by grants from the National Institute of General Medical Science (NIGMS) at the NIH (2P20 GM103480), National Institute of Dental and Craniofacial Research (NIDCR) at the NIH (1R21DE027516), NE LB606, and startup funds from the University of Nebraska Medical Center. Conflict of Interest: None. Publisher Copyright: © 2019 Elsevier Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/11

Y1 - 2019/11

N2 - Biomimetic and injectable nanofiber microspheres (NMs) could be ideal candidate for minimally invasive tissue repair. Herein, we report a facile approach to fabricate peptide-tethered NMs by combining electrospinning, electrospraying, and surface conjugation techniques. The composition and size of NMs can be tuned by varying the processing parameters during the fabrication. Further, bone morphogenic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) mimicking peptides have been successfully tethered onto poly(ε-caprolactone) (PCL):gelatin:(gelatin-methacryloyl) (GelMA)(1:0.5:0.5) NMs through photocrosslinking of the methacrylic group in GelMA and octenyl alanine (OCTAL) in the modified peptides. The BMP-2-OCTAL peptide-tethered NMs significantly promote osteogenic differentiation of bone marrow-derived stem cells (BMSCs). Moreover, human umbilical vein endothelial cells (HUVECs) seeded on VEGF mimicking peptide QK-OCTAL-tethered NMs significantly up-regulated vascular-specific proteins, leading to microvascularization. The strategy developed in this work holds great potential in developing a biomimetic and injectable carrier to efficiently direct cellular response (Osteogenesis and Angiogenesis) for tissue repair.

AB - Biomimetic and injectable nanofiber microspheres (NMs) could be ideal candidate for minimally invasive tissue repair. Herein, we report a facile approach to fabricate peptide-tethered NMs by combining electrospinning, electrospraying, and surface conjugation techniques. The composition and size of NMs can be tuned by varying the processing parameters during the fabrication. Further, bone morphogenic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) mimicking peptides have been successfully tethered onto poly(ε-caprolactone) (PCL):gelatin:(gelatin-methacryloyl) (GelMA)(1:0.5:0.5) NMs through photocrosslinking of the methacrylic group in GelMA and octenyl alanine (OCTAL) in the modified peptides. The BMP-2-OCTAL peptide-tethered NMs significantly promote osteogenic differentiation of bone marrow-derived stem cells (BMSCs). Moreover, human umbilical vein endothelial cells (HUVECs) seeded on VEGF mimicking peptide QK-OCTAL-tethered NMs significantly up-regulated vascular-specific proteins, leading to microvascularization. The strategy developed in this work holds great potential in developing a biomimetic and injectable carrier to efficiently direct cellular response (Osteogenesis and Angiogenesis) for tissue repair.

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KW - Osteogenic differentiation

KW - Peptides conjugation

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Tethering peptides onto biomimetic and injectable nanofiber microspheres to direct cellular response

Abstract

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