Tet-mediated formation of 5-hydroxymethylcytosine in RNA

Lijuan Fu; Candace R. Guerrero; Na Zhong; Nicholas J. Amato; Yunhua Liu; Shuo Liu; Qian Cai; Debin Ji; Seung Gi Jin; Laura J. Niedernhofer; Gerd P. Pfeifer; Guo Liang Xu; Yinsheng Wang

doi:10.1021/ja505305z

Tet-mediated formation of 5-hydroxymethylcytosine in RNA

Lijuan Fu
, Candace R. Guerrero
, Na Zhong
, Nicholas J. Amato
, Yunhua Liu
, Shuo Liu
, Qian Cai
, Debin Ji
, Seung Gi Jin
, Laura J. Niedernhofer
, Gerd P. Pfeifer
, Guo Liang Xu
, Yinsheng Wang

Biochemistry, Molecular Biology, and Biophysics (CBS)

Research output: Contribution to journal › Article › peer-review

351 Scopus citations

Abstract

Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

Original language	English (US)
Pages (from-to)	11582-11585
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	136
Issue number	33
DOIs	https://doi.org/10.1021/ja505305z
State	Published - Aug 20 2014

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SDG 3 Good Health and Well-being

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title = "Tet-mediated formation of 5-hydroxymethylcytosine in RNA",

abstract = "Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.",

author = "Lijuan Fu and Guerrero, \{Candace R.\} and Na Zhong and Amato, \{Nicholas J.\} and Yunhua Liu and Shuo Liu and Qian Cai and Debin Ji and Jin, \{Seung Gi\} and Niedernhofer, \{Laura J.\} and Pfeifer, \{Gerd P.\} and Xu, \{Guo Liang\} and Yinsheng Wang",

year = "2014",

month = aug,

day = "20",

doi = "10.1021/ja505305z",

language = "English (US)",

volume = "136",

pages = "11582--11585",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

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}

TY - JOUR

T1 - Tet-mediated formation of 5-hydroxymethylcytosine in RNA

AU - Fu, Lijuan

AU - Guerrero, Candace R.

AU - Zhong, Na

AU - Amato, Nicholas J.

AU - Liu, Yunhua

AU - Liu, Shuo

AU - Cai, Qian

AU - Ji, Debin

AU - Jin, Seung Gi

AU - Niedernhofer, Laura J.

AU - Pfeifer, Gerd P.

AU - Xu, Guo Liang

AU - Wang, Yinsheng

PY - 2014/8/20

Y1 - 2014/8/20

N2 - Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

AB - Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

UR - https://www.scopus.com/pages/publications/84906342396

UR - https://www.scopus.com/pages/publications/84906342396#tab=citedBy

U2 - 10.1021/ja505305z

DO - 10.1021/ja505305z

M3 - Article

C2 - 25073028

AN - SCOPUS:84906342396

SN - 0002-7863

VL - 136

SP - 11582

EP - 11585

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 33

ER -

Tet-mediated formation of 5-hydroxymethylcytosine in RNA

Abstract

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