Tet-mediated formation of 5-hydroxymethylcytosine in RNA

Lijuan Fu, Candace R. Guerrero, Na Zhong, Nicholas J. Amato, Yunhua Liu, Shuo Liu, Qian Cai, Debin Ji, Seung Gi Jin, Laura J. Niedernhofer, Gerd P. Pfeifer, Guo Liang Xu, Yinsheng Wang

Research output: Contribution to journalArticlepeer-review

138 Scopus citations


Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

Original languageEnglish (US)
Pages (from-to)11582-11585
Number of pages4
JournalJournal of the American Chemical Society
Issue number33
StatePublished - Aug 20 2014

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