Abstract
We give a detailed account on the enzymatic synthesis of RNA conjugates by T7 RNA polymerase using modified initiator nucleotides during transcription. Following two different routes, ternary conjugates of guanosine-5'-monophosphate, poly(ethylene glycol), and anthracene were synthesized via phosphoramidite intermediates and characterized by a variety of spectroscopic techniques. Up to a degree of polymerization n(PEG) of about 17, these conjugates were efficiently incorporated into RNA by T7 RNA polymerase at the 5'-termini, thereby giving access to RNA conjugates required for biochemical studies as well as for the exploration of the catalytic potential of ribonucleic acids. The resulting conjugates are intact and functional.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 371-378 |
| Number of pages | 8 |
| Journal | Bioconjugate Chemistry |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1999 |
Bibliographical note
Funding Information:We thank Shin-Jen Wu for the plasmids pHSC70(L399P) and pHSC70(R447G). We also are grateful to Dr. Ting-Fang Wang for comments on the manuscript. The work was supported by grants from the National Science Council (NSC-88-2311-B-001-056-B21, NSC-89-2311-B-001-078) and Academia Sinica of Taiwan, ROC.