Abstract
We give a detailed account on the enzymatic synthesis of RNA conjugates by T7 RNA polymerase using modified initiator nucleotides during transcription. Following two different routes, ternary conjugates of guanosine-5'-monophosphate, poly(ethylene glycol), and anthracene were synthesized via phosphoramidite intermediates and characterized by a variety of spectroscopic techniques. Up to a degree of polymerization n(PEG) of about 17, these conjugates were efficiently incorporated into RNA by T7 RNA polymerase at the 5'-termini, thereby giving access to RNA conjugates required for biochemical studies as well as for the exploration of the catalytic potential of ribonucleic acids. The resulting conjugates are intact and functional.
Original language | English (US) |
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Pages (from-to) | 371-378 |
Number of pages | 8 |
Journal | Bioconjugate Chemistry |
Volume | 10 |
Issue number | 3 |
DOIs | |
State | Published - 1999 |
Bibliographical note
Funding Information:We thank Shin-Jen Wu for the plasmids pHSC70(L399P) and pHSC70(R447G). We also are grateful to Dr. Ting-Fang Wang for comments on the manuscript. The work was supported by grants from the National Science Council (NSC-88-2311-B-001-056-B21, NSC-89-2311-B-001-078) and Academia Sinica of Taiwan, ROC.