Ternary conjugates of guanosine monophosphate as initiator nucleotides for the enzymatic synthesis of 5'-modified RNAs

Burckhard Seelig, Andres Jäschke

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

We give a detailed account on the enzymatic synthesis of RNA conjugates by T7 RNA polymerase using modified initiator nucleotides during transcription. Following two different routes, ternary conjugates of guanosine-5'-monophosphate, poly(ethylene glycol), and anthracene were synthesized via phosphoramidite intermediates and characterized by a variety of spectroscopic techniques. Up to a degree of polymerization n(PEG) of about 17, these conjugates were efficiently incorporated into RNA by T7 RNA polymerase at the 5'-termini, thereby giving access to RNA conjugates required for biochemical studies as well as for the exploration of the catalytic potential of ribonucleic acids. The resulting conjugates are intact and functional.

Original languageEnglish (US)
Pages (from-to)371-378
Number of pages8
JournalBioconjugate Chemistry
Volume10
Issue number3
DOIs
StatePublished - May 1 1999

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