Tension-depenent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast

Melissa K Gardner, Chad G. Pearson, Brian L. Sprague, Ted R. Zarzar, Kerry Bloom, E. D. Salmon, David J Odde

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

During metaphase in budding yeast mitosis, sister kinetochores are tethered to opposite poles and separated, stretching their intervening chromatin, by singly attached kinetochore microtubules (kMTs). Kinetochore movements are coupled to single microtubule plus-end polymerization/depolymerization at kinetochore attachment sites. Here, we use computer modeling to test possible mechanisms controlling chromosome alignment during yeast metaphase by simulating experiments that determine the 1) mean positions of kinetochore Cse4-GFP, 2) extent of oscillation of kinetochores during metaphase as measured by fluorescence recovery after photobleaching (FRAP) of kinetochore Cse4-GFP, 3) dynamics of kMTs as measured by FRAP of GFP-tubulin, and 4) mean positions of unreplicated chromosome kinetochores that lack pulling forces from a sister kinetochore. We rule out a number of possible models and find the best fit between theory and experiment when it is assumed that kinetochores sense both a spatial gradient that suppresses kMT catastrophe near the poles and attachment site tension that promotes kMT rescue at higher amounts of chromatin stretch.

Original languageEnglish (US)
Pages (from-to)3764-3775
Number of pages12
JournalMolecular Biology of the Cell
Volume16
Issue number8
DOIs
StatePublished - Aug 1 2005

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Kinetochores
Metaphase
Microtubules
Yeasts
Fluorescence Recovery After Photobleaching
Chromatin
Chromosomes
Saccharomycetales
Tubulin
Mitosis
Polymerization

Cite this

Tension-depenent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast. / Gardner, Melissa K; Pearson, Chad G.; Sprague, Brian L.; Zarzar, Ted R.; Bloom, Kerry; Salmon, E. D.; Odde, David J.

In: Molecular Biology of the Cell, Vol. 16, No. 8, 01.08.2005, p. 3764-3775.

Research output: Contribution to journalArticle

Gardner, Melissa K ; Pearson, Chad G. ; Sprague, Brian L. ; Zarzar, Ted R. ; Bloom, Kerry ; Salmon, E. D. ; Odde, David J. / Tension-depenent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 8. pp. 3764-3775.
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