Temporal network analysis identifies early physiological and transcriptomic indicators of mild drought in brassica rapa

Kathleen Greenham, Carmela Rosaria Guadagno, Malia A. Gehan, Todd C. Mockler, Cynthia Weinig, Brent E. Ewers, C. Robertson McClung

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The dynamics of local climates make development of agricultural strategies challenging. Yield improvement has progressed slowly, especially in drought-prone regions where annual crop production suffers from episodic aridity. Underlying drought responses are circadian and diel control of gene expression that regulate daily variations in metabolic and physiological pathways. To identify transcriptomic changes that occur in the crop Brassica rapa during initial perception of drought, we applied a co-expression network approach to associate rhythmic gene expression changes with physiological responses. Coupled analysis of transcriptome and physiological parameters over a two-day time course in control and drought-stressed plants provided temporal resolution necessary for correlation of network modules with dynamic changes in stomatal conductance, photosynthetic rate, and photosystem II efficiency. This approach enabled the identification of drought-responsive genes based on their differential rhythmic expression profiles in well-watered versus droughted networks and provided new insights into the dynamic physiological changes that occur during drought.

Original languageEnglish (US)
Article numbere29655
JournaleLife
Volume6
DOIs
StatePublished - Aug 18 2017
Externally publishedYes

Fingerprint Dive into the research topics of 'Temporal network analysis identifies early physiological and transcriptomic indicators of mild drought in brassica rapa'. Together they form a unique fingerprint.

  • Cite this

    Greenham, K., Guadagno, C. R., Gehan, M. A., Mockler, T. C., Weinig, C., Ewers, B. E., & McClung, C. R. (2017). Temporal network analysis identifies early physiological and transcriptomic indicators of mild drought in brassica rapa. eLife, 6, [e29655]. https://doi.org/10.7554/eLife.29655