Temporal and spatial requirements for Nodal-induced anterior mesendoderm and mesoderm in anterior neurulation

Ngawang Gonsar, Alicia Coughlin, Jessica A. Clay-Wright, Bethanie R. Borg, Lexy M. Kindt, Jennifer O Liang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Summary: Zebrafish with defective Nodal signaling have a phenotype analogous to the fatal human birth defect anencephaly, which is caused by an open anterior neural tube. Previous work in our laboratory found that anterior open neural tube phenotypes in Nodal signaling mutants were caused by lack of mesendodermal/mesodermal tissues. Defects in these mutants are already apparent at neural plate stage, before the neuroepithelium starts to fold into a tube. Consistent with this, we found that the requirement for Nodal signaling maps to mid-late blastula stages. This timing correlates with the timing of prechordal plate mesendoderm and anterior mesoderm induction, suggesting these tissues act to promote neurulation. To further identify tissues important for neurulation, we took advantage of the variable phenotypes in Nodal signaling-deficient sqt mutant and Lefty1-overexpressing embryos. Statistical analysis indicated a strong, positive correlation between a closed neural tube and presence of several mesendoderm/mesoderm-derived tissues (hatching glands, cephalic paraxial mesoderm, notochord, and head muscles). However, the neural tube was closed in a subset of embryos that lacked any one of these tissues. This suggests that several types of Nodal-induced mesendodermal/mesodermal precursors are competent to promote neurulation.

Original languageEnglish (US)
Pages (from-to)3-18
Number of pages16
JournalGenesis
Volume54
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Neurulation
Mesoderm
Neural Tube
Phenotype
Embryonic Structures
Head
Anencephaly
Blastula
Neural Plate
Notochord
Zebrafish
Muscles

Keywords

  • Anencephaly
  • Neural tube defects
  • Nodal
  • Zebrafish

Cite this

Temporal and spatial requirements for Nodal-induced anterior mesendoderm and mesoderm in anterior neurulation. / Gonsar, Ngawang; Coughlin, Alicia; Clay-Wright, Jessica A.; Borg, Bethanie R.; Kindt, Lexy M.; Liang, Jennifer O.

In: Genesis, Vol. 54, No. 1, 01.01.2016, p. 3-18.

Research output: Contribution to journalArticle

Gonsar, Ngawang ; Coughlin, Alicia ; Clay-Wright, Jessica A. ; Borg, Bethanie R. ; Kindt, Lexy M. ; Liang, Jennifer O. / Temporal and spatial requirements for Nodal-induced anterior mesendoderm and mesoderm in anterior neurulation. In: Genesis. 2016 ; Vol. 54, No. 1. pp. 3-18.
@article{9fd38654d67f44e3be2333437d48ef6f,
title = "Temporal and spatial requirements for Nodal-induced anterior mesendoderm and mesoderm in anterior neurulation",
abstract = "Summary: Zebrafish with defective Nodal signaling have a phenotype analogous to the fatal human birth defect anencephaly, which is caused by an open anterior neural tube. Previous work in our laboratory found that anterior open neural tube phenotypes in Nodal signaling mutants were caused by lack of mesendodermal/mesodermal tissues. Defects in these mutants are already apparent at neural plate stage, before the neuroepithelium starts to fold into a tube. Consistent with this, we found that the requirement for Nodal signaling maps to mid-late blastula stages. This timing correlates with the timing of prechordal plate mesendoderm and anterior mesoderm induction, suggesting these tissues act to promote neurulation. To further identify tissues important for neurulation, we took advantage of the variable phenotypes in Nodal signaling-deficient sqt mutant and Lefty1-overexpressing embryos. Statistical analysis indicated a strong, positive correlation between a closed neural tube and presence of several mesendoderm/mesoderm-derived tissues (hatching glands, cephalic paraxial mesoderm, notochord, and head muscles). However, the neural tube was closed in a subset of embryos that lacked any one of these tissues. This suggests that several types of Nodal-induced mesendodermal/mesodermal precursors are competent to promote neurulation.",
keywords = "Anencephaly, Neural tube defects, Nodal, Zebrafish",
author = "Ngawang Gonsar and Alicia Coughlin and Clay-Wright, {Jessica A.} and Borg, {Bethanie R.} and Kindt, {Lexy M.} and Liang, {Jennifer O}",
year = "2016",
month = "1",
day = "1",
doi = "10.1002/dvg.22908",
language = "English (US)",
volume = "54",
pages = "3--18",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Temporal and spatial requirements for Nodal-induced anterior mesendoderm and mesoderm in anterior neurulation

AU - Gonsar, Ngawang

AU - Coughlin, Alicia

AU - Clay-Wright, Jessica A.

AU - Borg, Bethanie R.

AU - Kindt, Lexy M.

AU - Liang, Jennifer O

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Summary: Zebrafish with defective Nodal signaling have a phenotype analogous to the fatal human birth defect anencephaly, which is caused by an open anterior neural tube. Previous work in our laboratory found that anterior open neural tube phenotypes in Nodal signaling mutants were caused by lack of mesendodermal/mesodermal tissues. Defects in these mutants are already apparent at neural plate stage, before the neuroepithelium starts to fold into a tube. Consistent with this, we found that the requirement for Nodal signaling maps to mid-late blastula stages. This timing correlates with the timing of prechordal plate mesendoderm and anterior mesoderm induction, suggesting these tissues act to promote neurulation. To further identify tissues important for neurulation, we took advantage of the variable phenotypes in Nodal signaling-deficient sqt mutant and Lefty1-overexpressing embryos. Statistical analysis indicated a strong, positive correlation between a closed neural tube and presence of several mesendoderm/mesoderm-derived tissues (hatching glands, cephalic paraxial mesoderm, notochord, and head muscles). However, the neural tube was closed in a subset of embryos that lacked any one of these tissues. This suggests that several types of Nodal-induced mesendodermal/mesodermal precursors are competent to promote neurulation.

AB - Summary: Zebrafish with defective Nodal signaling have a phenotype analogous to the fatal human birth defect anencephaly, which is caused by an open anterior neural tube. Previous work in our laboratory found that anterior open neural tube phenotypes in Nodal signaling mutants were caused by lack of mesendodermal/mesodermal tissues. Defects in these mutants are already apparent at neural plate stage, before the neuroepithelium starts to fold into a tube. Consistent with this, we found that the requirement for Nodal signaling maps to mid-late blastula stages. This timing correlates with the timing of prechordal plate mesendoderm and anterior mesoderm induction, suggesting these tissues act to promote neurulation. To further identify tissues important for neurulation, we took advantage of the variable phenotypes in Nodal signaling-deficient sqt mutant and Lefty1-overexpressing embryos. Statistical analysis indicated a strong, positive correlation between a closed neural tube and presence of several mesendoderm/mesoderm-derived tissues (hatching glands, cephalic paraxial mesoderm, notochord, and head muscles). However, the neural tube was closed in a subset of embryos that lacked any one of these tissues. This suggests that several types of Nodal-induced mesendodermal/mesodermal precursors are competent to promote neurulation.

KW - Anencephaly

KW - Neural tube defects

KW - Nodal

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=84956594478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84956594478&partnerID=8YFLogxK

U2 - 10.1002/dvg.22908

DO - 10.1002/dvg.22908

M3 - Article

C2 - 26528772

AN - SCOPUS:84956594478

VL - 54

SP - 3

EP - 18

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 1

ER -