Temperature-sensitive gel for virus concentration from urine

S. Maheshkumar; Richard B. Peterson; Sagar M. Goyal

doi:10.1016/0166-0934(89)90087-6

Temperature-sensitive gel for virus concentration from urine

S. Maheshkumar, Richard B. Peterson, Sagar M. Goyal

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Cross-linked, partially hydrolyzed polyacrylamide gels (temperature-sensitive gels) with the property to swell at 4°C and collapse at higher temperatures (> 19°C) were used to concentrate bacteriophage T-2 from urine. Samples of urine, 50 ml, seeded with bacteriophage T-2 were reduced to approximately 5 ml, with an average virus recovery of 53%. Subsequent experiments with feline cell-associated herpes virus resulted in a 6-fold decrease in volume with 54% virus recovery. The gel could be used repeatedly without any loss in efficiency.

Original language	English (US)
Pages (from-to)	41-46
Number of pages	6
Journal	Journal of Virological Methods
Volume	23
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(89)90087-6
State	Published - Jan 1989

Bibliographical note

Funding Information:
This researchw as supportedi n part by grantsf rom U.S. Departmento f Commerce through the National Sea Grant Program and from U.S. Departmento f Agriculture through a CSRS grant.

Keywords

Bacteriophage T-2
Feline herpesvirus
Temperature-sensitive gel
Virus concentration
Virus in urine

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10.1016/0166-0934(89)90087-6

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abstract = "Cross-linked, partially hydrolyzed polyacrylamide gels (temperature-sensitive gels) with the property to swell at 4°C and collapse at higher temperatures (> 19°C) were used to concentrate bacteriophage T-2 from urine. Samples of urine, 50 ml, seeded with bacteriophage T-2 were reduced to approximately 5 ml, with an average virus recovery of 53%. Subsequent experiments with feline cell-associated herpes virus resulted in a 6-fold decrease in volume with 54% virus recovery. The gel could be used repeatedly without any loss in efficiency.",

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AU - Maheshkumar, S.

AU - Peterson, Richard B.

AU - Goyal, Sagar M.

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N2 - Cross-linked, partially hydrolyzed polyacrylamide gels (temperature-sensitive gels) with the property to swell at 4°C and collapse at higher temperatures (> 19°C) were used to concentrate bacteriophage T-2 from urine. Samples of urine, 50 ml, seeded with bacteriophage T-2 were reduced to approximately 5 ml, with an average virus recovery of 53%. Subsequent experiments with feline cell-associated herpes virus resulted in a 6-fold decrease in volume with 54% virus recovery. The gel could be used repeatedly without any loss in efficiency.

AB - Cross-linked, partially hydrolyzed polyacrylamide gels (temperature-sensitive gels) with the property to swell at 4°C and collapse at higher temperatures (> 19°C) were used to concentrate bacteriophage T-2 from urine. Samples of urine, 50 ml, seeded with bacteriophage T-2 were reduced to approximately 5 ml, with an average virus recovery of 53%. Subsequent experiments with feline cell-associated herpes virus resulted in a 6-fold decrease in volume with 54% virus recovery. The gel could be used repeatedly without any loss in efficiency.

KW - Bacteriophage T-2

KW - Feline herpesvirus

KW - Temperature-sensitive gel

KW - Virus concentration

KW - Virus in urine

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Temperature-sensitive gel for virus concentration from urine

Abstract

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Keywords

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