Temperature-sensitive gel for virus concentration from urine

S. Maheshkumar, Richard B. Peterson, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review

Abstract

Cross-linked, partially hydrolyzed polyacrylamide gels (temperature-sensitive gels) with the property to swell at 4°C and collapse at higher temperatures (> 19°C) were used to concentrate bacteriophage T-2 from urine. Samples of urine, 50 ml, seeded with bacteriophage T-2 were reduced to approximately 5 ml, with an average virus recovery of 53%. Subsequent experiments with feline cell-associated herpes virus resulted in a 6-fold decrease in volume with 54% virus recovery. The gel could be used repeatedly without any loss in efficiency.

Original languageEnglish (US)
Pages (from-to)41-46
Number of pages6
JournalJournal of Virological Methods
Volume23
Issue number1
DOIs
StatePublished - Jan 1989

Keywords

  • Bacteriophage T-2
  • Feline herpesvirus
  • Temperature-sensitive gel
  • Virus concentration
  • Virus in urine

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