Temperature regulates bacterial protein production: Possible role in rosacea

Mark V Dahl, Amy J. Ross, Patrick M. Schlievert

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Facial skin temperature is higher for patients with rosacea. Papules and pustules might arise because bacteria behave differently at these warmer temperatures. We sought to: (1) compare bacteria from facial skin of patients with rosacea with that of control subjects; and (2) grow these bacteria at 30°C and 37°C to compare growth curves and secreted proteins. Bacteria isolated from pustules/skin surfaces of patients with rosacea and skin surfaces of control subjects were identified and cultured at 37°C and 30°C. Secreted proteins were separated by electrophoresis. We found that Staphylococcus epidermidis isolated from patients with rosacea was consistently β-hemolytic, whereas that from control subjects were nonhemolytic. Bacteria from patients with rosacea grew at the same rate and to the same stationary phase whether cultured at 37°C or 30°C. Isolates from patients with rosacea secreted more proteins, and generally more of each protein at 37°C compared with 30°C. In conclusion, bacteria isolated from patients with rosacea secrete different proteins and different amounts of protein at different temperatures.

Original languageEnglish (US)
Pages (from-to)266-272
Number of pages7
JournalJournal of the American Academy of Dermatology
Volume50
Issue number2
DOIs
StatePublished - Feb 2004

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