TY - JOUR
T1 - Targeting of herpesvirus capsid transport in axons is coupled to association with specific sets of tegument proteins
AU - Luxton, G. W.Gant
AU - Haverlock, Sarah
AU - Coller, Kelly Elizabeth
AU - Antinone, Sarah Elizabeth
AU - Pincetic, Andrew
AU - Smith, Gregory Allan
PY - 2005/4/19
Y1 - 2005/4/19
N2 - The capsids of neurotropic herpesviruses have the remarkable ability to move in specific directions within axons. By modulating bidirectional capsid transport to favor either retrograde (minus-end) or anterograde (plus-end) motion, these viruses travel to sensory ganglia or peripheral tissue at specific stages of infection. By using correlative motion analysis to simultaneously monitor the trafficking of distinct viral proteins in living neurons, we demonstrate that viral "tegument" proteins are complexed to capsids moving in axons. The removal of a subset of tegument proteins from capsids invariably preceded retrograde transport to the cell body in sensory ganglia, whereas addition of these proteins was coupled to anterograde transport of progeny capsids to the distal axon. Although capsid transport never occurred without associated tegument proteins, anterograde-specific tegument proteins were competent to travel to the distal axon independent of capsids. These findings are compatible with a model of viral bidirectional transport in which tegument proteins direct capsid traffic to specific intracellular locations during the infectious cycle.
AB - The capsids of neurotropic herpesviruses have the remarkable ability to move in specific directions within axons. By modulating bidirectional capsid transport to favor either retrograde (minus-end) or anterograde (plus-end) motion, these viruses travel to sensory ganglia or peripheral tissue at specific stages of infection. By using correlative motion analysis to simultaneously monitor the trafficking of distinct viral proteins in living neurons, we demonstrate that viral "tegument" proteins are complexed to capsids moving in axons. The removal of a subset of tegument proteins from capsids invariably preceded retrograde transport to the cell body in sensory ganglia, whereas addition of these proteins was coupled to anterograde transport of progeny capsids to the distal axon. Although capsid transport never occurred without associated tegument proteins, anterograde-specific tegument proteins were competent to travel to the distal axon independent of capsids. These findings are compatible with a model of viral bidirectional transport in which tegument proteins direct capsid traffic to specific intracellular locations during the infectious cycle.
KW - Neuron
KW - Virus
UR - http://www.scopus.com/inward/record.url?scp=17644404401&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17644404401&partnerID=8YFLogxK
U2 - 10.1073/pnas.0500803102
DO - 10.1073/pnas.0500803102
M3 - Article
C2 - 15795370
AN - SCOPUS:17644404401
SN - 0027-8424
VL - 102
SP - 5832
EP - 5837
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 16
ER -