Targeting DNA double-strand breaks with TAL effector nucleases

Michelle Christian, Tomas Cermak, Erin L. Doyle, Clarice Schmidt, Feng Zhang, Aaron Hummel, Adam J. Bogdanove, Daniel F. Voytas

Research output: Contribution to journalArticlepeer-review

1520 Scopus citations


Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.

Original languageEnglish (US)
Pages (from-to)756-761
Number of pages6
Issue number2
StatePublished - Oct 2010


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