Targeted Analysis of Chromatin Events (TACE)

Jason Sims, Changbin Chen, Peter Schlögelhofer, Marie Therese Kurzbauer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Visualization of meiotic chromatin from pollen mother cells has become an essential technique to study meiosis in the model plant Arabidopsis thaliana. Here we present an advanced cytogenetic method that combines improved immunocytology with chromosome painting, thereby generating a tool to quantitatively analyze localization of proteins to any given genomic region. Proteins involved in different processes such as DNA double-strand break formation and recombinational repair can be visualized on meiotic chromatin with the additional feature of assessing their abundance at specific chromosomal locations.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages47-58
Number of pages12
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2061
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Arabidopsis thaliana
  • Chromosome painting
  • FISH
  • Immunocytology
  • Meiosis

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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  • Cite this

    Sims, J., Chen, C., Schlögelhofer, P., & Kurzbauer, M. T. (2020). Targeted Analysis of Chromatin Events (TACE). In Methods in Molecular Biology (pp. 47-58). (Methods in Molecular Biology; Vol. 2061). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9818-0_5