Targeted Analysis of Chromatin Events (TACE)

Jason Sims, Changbin Chen, Peter Schlögelhofer, Marie Therese Kurzbauer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Visualization of meiotic chromatin from pollen mother cells has become an essential technique to study meiosis in the model plant Arabidopsis thaliana. Here we present an advanced cytogenetic method that combines improved immunocytology with chromosome painting, thereby generating a tool to quantitatively analyze localization of proteins to any given genomic region. Proteins involved in different processes such as DNA double-strand break formation and recombinational repair can be visualized on meiotic chromatin with the additional feature of assessing their abundance at specific chromosomal locations.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages47-58
Number of pages12
Volume2061
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2061
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Funding Information:
We thank the European Union (FP7-ITN 560 606956), the University of Vienna (I031-B), and the Austrian Science Fund (SFB F3408; P18036) for funding (J.S., P.S., and M.T.K.). C.C. is supported by the National Science Foundation (IOS:1025881 and IOS:1546792) and the Grant-in-Aid program at the University of Minnesota. We furthermore thank Chris Franklin for providing the ASY1 antibody and Sue Armstrong, James Higgins, and Katja Schneider for very helpful technical demonstrations and discussions.

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

  • Arabidopsis thaliana
  • Chromosome painting
  • FISH
  • Immunocytology
  • Meiosis

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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