TALEN-Mediated mutagenesis and genome editing

Alvin C.H. Ma; Yi Chen; Patrick R. Blackburn; Stephen C. Ekker

doi:10.1007/978-1-4939-3771-4_2

TALEN-Mediated mutagenesis and genome editing

Alvin C.H. Ma, Yi Chen, Patrick R. Blackburn, Stephen C. Ekker

Chancellor's Office (Rochester)

Research output: Chapter in Book/Report/Conference proceeding › Chapter

21 Scopus citations

Abstract

Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	17-30
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-4939-3771-4_2
State	Published - Jan 1 2016

Publication series

Name	Methods in Molecular Biology
Volume	1451
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

Customized nucleases
FusX
Genome editing
Golden Gate
TALEN
Zebrafish

Publisher link

10.1007/978-1-4939-3771-4_2

http://europepmc.org/articles/pmc5777227

Find It

Find It at U of M Libraries

Cite this

@inbook{4c9cd67b50684003a830ea844e690736,

title = "TALEN-Mediated mutagenesis and genome editing",

abstract = "Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.",

keywords = "Customized nucleases, FusX, Genome editing, Golden Gate, TALEN, Zebrafish",

author = "Ma, {Alvin C.H.} and Yi Chen and Blackburn, {Patrick R.} and Ekker, {Stephen C.}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",

year = "2016",

month = jan,

day = "1",

doi = "10.1007/978-1-4939-3771-4_2",

language = "English (US)",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "17--30",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - TALEN-Mediated mutagenesis and genome editing

AU - Ma, Alvin C.H.

AU - Chen, Yi

AU - Blackburn, Patrick R.

AU - Ekker, Stephen C.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.

AB - Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.

KW - Customized nucleases

KW - FusX

KW - Genome editing

KW - Golden Gate

KW - TALEN

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=84981484538&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84981484538&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3771-4_2

DO - 10.1007/978-1-4939-3771-4_2

M3 - Chapter

C2 - 27464798

AN - SCOPUS:84981484538

T3 - Methods in Molecular Biology

SP - 17

EP - 30

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

TALEN-Mediated mutagenesis and genome editing

Abstract

Publication series

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this