TALEN-Mediated mutagenesis and genome editing

Alvin C.H. Ma, Yi Chen, Patrick R. Blackburn, Stephen C. Ekker

    Research output: Chapter in Book/Report/Conference proceedingChapter

    12 Scopus citations

    Abstract

    Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages17-30
    Number of pages14
    DOIs
    StatePublished - Jan 1 2016

    Publication series

    NameMethods in Molecular Biology
    Volume1451
    ISSN (Print)1064-3745

    Keywords

    • Customized nucleases
    • FusX
    • Genome editing
    • Golden Gate
    • TALEN
    • Zebrafish

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