TALEN-Mediated mutagenesis and genome editing

Alvin C.H. Ma, Yi Chen, Patrick R. Blackburn, Stephen C. Ekker

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations

Abstract

Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages17-30
Number of pages14
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1451
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

  • Customized nucleases
  • FusX
  • Genome editing
  • Golden Gate
  • TALEN
  • Zebrafish

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