TALEN-mediated editing of the mouse y chromosome

Haoyi Wang; Yueh Chiang Hu; Styliani Markoulaki; G. Grant Welstead; Albert W. Cheng; Chikdu S. Shivalila; Tatyana Pyntikova; Daniel B. Dadon; Daniel F. Voytas; Adam J. Bogdanove; David C. Page; Rudolf Jaenisch

doi:10.1038/nbt.2595

TALEN-mediated editing of the mouse y chromosome

Haoyi Wang, Yueh Chiang Hu, Styliani Markoulaki, G. Grant Welstead, Albert W. Cheng, Chikdu S. Shivalila, Tatyana Pyntikova, Daniel B. Dadon, Daniel F. Voytas, Adam J. Bogdanove, David C. Page, Rudolf Jaenisch

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88 Scopus citations

Abstract

The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes - Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.

Original language	English (US)
Pages (from-to)	530-532
Number of pages	3
Journal	Nature biotechnology
Volume	31
Issue number	6
DOIs	https://doi.org/10.1038/nbt.2595
State	Published - Jun 2013

Bibliographical note

Funding Information:
We thank K. Ganz and R. Flannery for support with animal care and experiments. We thank Jaenisch and Page laboratory members for helpful discussions on the manuscript. A.W.C. is supported by a Croucher scholarship. D.C.P. and Y.-C.H. were supported by Howard Hughes Medical Institute. R.J., H.W., S.M., G.G.W., C.S.S. and A.W.C. were supported by US National Institutes of Health grants R37-HD045022 and R01-CA084198. T.P. was supported by US National Institutes of Health grant R01-HG000257.

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title = "TALEN-mediated editing of the mouse y chromosome",

abstract = "The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes - Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.",

author = "Haoyi Wang and Hu, {Yueh Chiang} and Styliani Markoulaki and Welstead, {G. Grant} and Cheng, {Albert W.} and Shivalila, {Chikdu S.} and Tatyana Pyntikova and Dadon, {Daniel B.} and Voytas, {Daniel F.} and Bogdanove, {Adam J.} and Page, {David C.} and Rudolf Jaenisch",

note = "Funding Information: We thank K. Ganz and R. Flannery for support with animal care and experiments. We thank Jaenisch and Page laboratory members for helpful discussions on the manuscript. A.W.C. is supported by a Croucher scholarship. D.C.P. and Y.-C.H. were supported by Howard Hughes Medical Institute. R.J., H.W., S.M., G.G.W., C.S.S. and A.W.C. were supported by US National Institutes of Health grants R37-HD045022 and R01-CA084198. T.P. was supported by US National Institutes of Health grant R01-HG000257.",

year = "2013",

month = jun,

doi = "10.1038/nbt.2595",

language = "English (US)",

volume = "31",

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AU - Wang, Haoyi

AU - Hu, Yueh Chiang

AU - Markoulaki, Styliani

AU - Welstead, G. Grant

AU - Cheng, Albert W.

AU - Shivalila, Chikdu S.

AU - Pyntikova, Tatyana

AU - Dadon, Daniel B.

AU - Voytas, Daniel F.

AU - Bogdanove, Adam J.

AU - Page, David C.

AU - Jaenisch, Rudolf

N1 - Funding Information: We thank K. Ganz and R. Flannery for support with animal care and experiments. We thank Jaenisch and Page laboratory members for helpful discussions on the manuscript. A.W.C. is supported by a Croucher scholarship. D.C.P. and Y.-C.H. were supported by Howard Hughes Medical Institute. R.J., H.W., S.M., G.G.W., C.S.S. and A.W.C. were supported by US National Institutes of Health grants R37-HD045022 and R01-CA084198. T.P. was supported by US National Institutes of Health grant R01-HG000257.

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TALEN-mediated editing of the mouse y chromosome

Abstract

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