TALEN-mediated editing of the mouse y chromosome

Haoyi Wang, Yueh Chiang Hu, Styliani Markoulaki, G. Grant Welstead, Albert W. Cheng, Chikdu S. Shivalila, Tatyana Pyntikova, Daniel B. Dadon, Daniel F. Voytas, Adam J. Bogdanove, David C. Page, Rudolf Jaenisch

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes - Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.

Original languageEnglish (US)
Pages (from-to)530-532
Number of pages3
JournalNature biotechnology
Volume31
Issue number6
DOIs
StatePublished - Jun 2013

Bibliographical note

Funding Information:
We thank K. Ganz and R. Flannery for support with animal care and experiments. We thank Jaenisch and Page laboratory members for helpful discussions on the manuscript. A.W.C. is supported by a Croucher scholarship. D.C.P. and Y.-C.H. were supported by Howard Hughes Medical Institute. R.J., H.W., S.M., G.G.W., C.S.S. and A.W.C. were supported by US National Institutes of Health grants R37-HD045022 and R01-CA084198. T.P. was supported by US National Institutes of Health grant R01-HG000257.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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