Tailor-made mutations in arabidopsis using zinc finger nucleases

Yiping Qi, Colby G. Starker, Feng Zhang, Nicholas J. Baltes, Daniel F. Voytas

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Zinc finger nucleases (ZFNs) are proteins engineered to make site-specific double-strand breaks (DSBs) in a DNA sequence of interest. Imprecise repair of the ZFN-induced DSBs by the nonhomologous end-joining (NHEJ) pathway results in a spectrum of mutations, such as nucleotide substitutions, insertions, and deletions. Here we describe a method for targeted mutagenesis in Arabidopsis with ZFNs, which are engineered by context-dependent assembly (CoDA). This ZFN-induced mutagenesis method is an alternative to other currently available gene knockout or knockdown technologies and is useful for reverse genetic studies.

Original languageEnglish (US)
Title of host publicationArabidopsis Protocols
PublisherHumana Press Inc.
Pages193-209
Number of pages17
ISBN (Print)9781627035798
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Molecular Biology
Volume1062
ISSN (Print)1064-3745

Keywords

  • Arabidopsis
  • CoDA
  • Mutagenesis
  • NHEJ
  • ZFN

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