Synthetic peptides derived from the basement membrane protein laminin promote heparin binding and human keratinocyte adhesion

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, normal human keratinocytes were isolated from neonatal foreskins and cultured in nutrient keratinocyte basal medium (Clonetics) supplemented with 5 × 10-7 M hydrocortisone, 5 μg/ml insulin, 10 ng/ml epidermal growth factor, and 140 μg/ml protein of bovine pituitary extract. Laminin was isolated from the murine Engelbreth-Holm-Swarm tumor and -20′$mer peptides were chemically synthesized and HPLC-purified. It is shown that the peptides have potential uses in wound healing and biocompatibility, whereby surfaces or wound beds themselves, treated with the peptides may promote keratinocyte cell adhesion and reepithelialization.

Original languageEnglish (US)
Title of host publicationTransactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium
PublisherPubl by Soc for Biomaterials
Number of pages1
Volume14
StatePublished - Jan 1 1991
Event17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium - Scottsdale, AZ, USA
Duration: May 1 1991May 5 1991

Other

Other17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium
CityScottsdale, AZ, USA
Period5/1/915/5/91

Fingerprint

Laminin
Peptides
Heparin
Membrane Proteins
Adhesion
Proteins
Cortisol
Insulin
Cell adhesion
Biocompatibility
Epidermal Growth Factor
Nutrients
Hydrocortisone
Tumors
Keratinocytes
Basement Membrane

Cite this

Skubitz, A. P., Wilke, M. S., & Furcht, L. T. (1991). Synthetic peptides derived from the basement membrane protein laminin promote heparin binding and human keratinocyte adhesion. In Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium (Vol. 14). Publ by Soc for Biomaterials.

Synthetic peptides derived from the basement membrane protein laminin promote heparin binding and human keratinocyte adhesion. / Skubitz, Amy P; Wilke, M. S.; Furcht, Leo T.

Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. Vol. 14 Publ by Soc for Biomaterials, 1991.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Skubitz, AP, Wilke, MS & Furcht, LT 1991, Synthetic peptides derived from the basement membrane protein laminin promote heparin binding and human keratinocyte adhesion. in Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. vol. 14, Publ by Soc for Biomaterials, 17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium, Scottsdale, AZ, USA, 5/1/91.
Skubitz AP, Wilke MS, Furcht LT. Synthetic peptides derived from the basement membrane protein laminin promote heparin binding and human keratinocyte adhesion. In Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. Vol. 14. Publ by Soc for Biomaterials. 1991
Skubitz, Amy P ; Wilke, M. S. ; Furcht, Leo T. / Synthetic peptides derived from the basement membrane protein laminin promote heparin binding and human keratinocyte adhesion. Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. Vol. 14 Publ by Soc for Biomaterials, 1991.
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