Synthetic fibronectin peptide exerts neuroprotective effects on transient focal brain ischemia in rats

Li Ru Zhao, Steve Spellman, Jonggul Kim, Wei Ming Duan, James B. McCarthy, Walter C. Low

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Leukocytes have been investigated during the past decade for their roles in secondary tissue damage after ischemia/reperfusion injury. Peptide PRARIY, a synthetic fibronectin peptide, has shown an anti-adhesion effect in in vitro studies. Previous studies have demonstrated that anti-adhesion agents lead to reductions in apoptosis. The purpose of the present study was to determine whether the peptide PRARIY displays anti-inflammatory, anti-apoptotic, and neuroprotective effects following transient focal brain ischemia in rats. Twenty-six male Sprague-Dawley rats (300-350 g) were randomly divided into three groups: phosphate-buffered saline (PBS) controls, PRARI controls, and PRARIY treatments. The right middle cerebral artery was transiently occluded using a 4-0 nylon suture. One hour later, the occluder was withdrawn, and reperfusion was maintained for 48 h. Immediately after reperfusion, the peptides (20 mg/kg, dissolved in PBS) and the same volume of PBS were continuously infused through the right external carotid artery using an osmotic minipump for 24 h. Neurological deficits were examined at 3, 24, and 48 h after ischemia. Forty-eight hours after reperfusion, the rats were sacrificed for determining infarction size, leukocyte infiltration, and apoptosis in the ischemia area. Unexpectedly, PRARIY did not influence leukocyte infiltration. However, PRARIY-treated rats showed significantly functional outcome, reduction of infarction size, decrease of TUNEL positive cells, and increase of Bcl-2 (anti-apoptotic protein) positive cells in the ischemic areas when compared to the controls. These data indicate that the peptide PRARIY exerts its neuroprotective effects via supporting neural cell survival rather than anti-leukocyte recruitment following brain ischemia/reperfusion injury.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalBrain Research
Issue number1
StatePublished - Aug 23 2005

Bibliographical note

Funding Information:
We thank Bruce Lindgren from Division of Biostatistics, University of Minnesota, for his helpful suggestions about the statistical analyses. This work was supported by the Lyle French Neurosurgery Research Fund.


  • Anti-adhesion
  • Apoptosis
  • Fibronectin
  • Ischemia
  • MCAO


Dive into the research topics of 'Synthetic fibronectin peptide exerts neuroprotective effects on transient focal brain ischemia in rats'. Together they form a unique fingerprint.

Cite this