Synthesis, purification and crystallization of guanine-rich RNA oligonucleotides

Baocheng Pan, Ke Shi, Muttaiya Sundaralingam

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Guanine-rich RNA oligonucleotides display many novel structural motifs in recent crystal structures. Here we describe the procedures of the chemical synthesis and the purification of such RNA molecules that are suitable for X-ray crystallographic studies. Modifications of the previous purification methods allow us to obtain better yields in shorter time. We also provide 24 screening conditions that are very effective in crystallization of the guanine-rich RNA oligonucleotides. Optimal crystallization conditions are usually achieved by adjustment of the concentration of the metal ions and pH of the buffer. Crystals obtained by this method usually diffract to high resolution.

Original languageEnglish (US)
Pages (from-to)257-262
Number of pages6
JournalBiological Procedures Online
Issue number1
StatePublished - Nov 22 2004


  • Crystallization
  • Isolation and purification
  • Oligonucleotides


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