Synthesis of mesoporous silica nanoparticle-oxaliplatin conjugates for improved anticancer drug delivery

Hongyan He; Haihua Xiao; Huihui Kuang; Zhigang Xie; Xuesi Chen; Xiabin Jing; Yubin Huang

doi:10.1016/j.colsurfb.2014.02.014

Synthesis of mesoporous silica nanoparticle-oxaliplatin conjugates for improved anticancer drug delivery

Hongyan He, Haihua Xiao, Huihui Kuang, Zhigang Xie, Xuesi Chen, Xiabin Jing, Yubin Huang

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Mesoporous silica nanoparticles (MSN) with 1,2-bidentate carboxyl groups on the surface reacted with 1,2-diaminecyclohexano platinum(II) dinitrate (DACH-Pt-(NO₃)₂) which is an active anticancer species of clinic relevant oxaliplatin to form MSN-Pt. The modification of the parent particles was monitored by ¹³C, ²⁹Si solid-state NMR, X-ray measurements (XRD) and Fourier transform infrared spectroscopy (FT-IR). After loading with platinum drugs, MSN-Pt exhibited two strong Pt_4f signals as indicated by X-ray photoelectron spectroscopy (XPS). The platinum content in the conjugates was calculated to be 9.7% according to ICP-MS measurements. Confocal laser scanning microscopy (CLSM) displayed that MSN-Pt were uptaken fast by HepG-2 cells and concentrated within endosomes and lysosomes. In vitro MTT assay of MSN-Pt demonstrated an improved cytotoxicity against HepG-2 cells than that of free oxaliplatin. This is due to the fact that MSN-Pt expressed higher platinum intracellular uptake and more DNA binding (Pt-DNA adducts) than free oxaliplatin. Hence this work highlighted that the platinum loaded MSN nanoparticles could be a promising future intelligent drug delivery system.

Original language	English (US)
Pages (from-to)	75-81
Number of pages	7
Journal	Colloids and Surfaces B: Biointerfaces
Volume	117
DOIs	https://doi.org/10.1016/j.colsurfb.2014.02.014
State	Published - May 1 2014

Bibliographical note

Funding Information:
The authors would like to thank the financial support from National Natural Science Foundation of China (Nos. 51321062 , 51273194 and 21174143 ), the Ministry of Science and Technology of China (973 Project, No. 2009CB930102; 863 Project, No. 2012AA021900), “100 Talents Program” of the Chinese Academy of Sciences (No. KGCX2-YW-802 ), and Jilin Provincial Science and Technology Department (No. 20100588 ).

Keywords

Conjugates
Drug delivery
Mesoporous silica nanoparticles (MSN)
Oxaliplatin
Thiol-ene click

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.colsurfb.2014.02.014

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@article{4ef65c16d384407996e2c6f25600611b,

title = "Synthesis of mesoporous silica nanoparticle-oxaliplatin conjugates for improved anticancer drug delivery",

abstract = "Mesoporous silica nanoparticles (MSN) with 1,2-bidentate carboxyl groups on the surface reacted with 1,2-diaminecyclohexano platinum(II) dinitrate (DACH-Pt-(NO3)2) which is an active anticancer species of clinic relevant oxaliplatin to form MSN-Pt. The modification of the parent particles was monitored by 13C, 29Si solid-state NMR, X-ray measurements (XRD) and Fourier transform infrared spectroscopy (FT-IR). After loading with platinum drugs, MSN-Pt exhibited two strong Pt4f signals as indicated by X-ray photoelectron spectroscopy (XPS). The platinum content in the conjugates was calculated to be 9.7% according to ICP-MS measurements. Confocal laser scanning microscopy (CLSM) displayed that MSN-Pt were uptaken fast by HepG-2 cells and concentrated within endosomes and lysosomes. In vitro MTT assay of MSN-Pt demonstrated an improved cytotoxicity against HepG-2 cells than that of free oxaliplatin. This is due to the fact that MSN-Pt expressed higher platinum intracellular uptake and more DNA binding (Pt-DNA adducts) than free oxaliplatin. Hence this work highlighted that the platinum loaded MSN nanoparticles could be a promising future intelligent drug delivery system.",

keywords = "Conjugates, Drug delivery, Mesoporous silica nanoparticles (MSN), Oxaliplatin, Thiol-ene click",

author = "Hongyan He and Haihua Xiao and Huihui Kuang and Zhigang Xie and Xuesi Chen and Xiabin Jing and Yubin Huang",

note = "Funding Information: The authors would like to thank the financial support from National Natural Science Foundation of China (Nos. 51321062 , 51273194 and 21174143 ), the Ministry of Science and Technology of China (973 Project, No. 2009CB930102; 863 Project, No. 2012AA021900), “100 Talents Program” of the Chinese Academy of Sciences (No. KGCX2-YW-802 ), and Jilin Provincial Science and Technology Department (No. 20100588 ).",

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doi = "10.1016/j.colsurfb.2014.02.014",

language = "English (US)",

volume = "117",

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T1 - Synthesis of mesoporous silica nanoparticle-oxaliplatin conjugates for improved anticancer drug delivery

AU - He, Hongyan

AU - Xiao, Haihua

AU - Kuang, Huihui

AU - Xie, Zhigang

AU - Chen, Xuesi

AU - Jing, Xiabin

AU - Huang, Yubin

N1 - Funding Information: The authors would like to thank the financial support from National Natural Science Foundation of China (Nos. 51321062 , 51273194 and 21174143 ), the Ministry of Science and Technology of China (973 Project, No. 2009CB930102; 863 Project, No. 2012AA021900), “100 Talents Program” of the Chinese Academy of Sciences (No. KGCX2-YW-802 ), and Jilin Provincial Science and Technology Department (No. 20100588 ).

PY - 2014/5/1

Y1 - 2014/5/1

N2 - Mesoporous silica nanoparticles (MSN) with 1,2-bidentate carboxyl groups on the surface reacted with 1,2-diaminecyclohexano platinum(II) dinitrate (DACH-Pt-(NO3)2) which is an active anticancer species of clinic relevant oxaliplatin to form MSN-Pt. The modification of the parent particles was monitored by 13C, 29Si solid-state NMR, X-ray measurements (XRD) and Fourier transform infrared spectroscopy (FT-IR). After loading with platinum drugs, MSN-Pt exhibited two strong Pt4f signals as indicated by X-ray photoelectron spectroscopy (XPS). The platinum content in the conjugates was calculated to be 9.7% according to ICP-MS measurements. Confocal laser scanning microscopy (CLSM) displayed that MSN-Pt were uptaken fast by HepG-2 cells and concentrated within endosomes and lysosomes. In vitro MTT assay of MSN-Pt demonstrated an improved cytotoxicity against HepG-2 cells than that of free oxaliplatin. This is due to the fact that MSN-Pt expressed higher platinum intracellular uptake and more DNA binding (Pt-DNA adducts) than free oxaliplatin. Hence this work highlighted that the platinum loaded MSN nanoparticles could be a promising future intelligent drug delivery system.

AB - Mesoporous silica nanoparticles (MSN) with 1,2-bidentate carboxyl groups on the surface reacted with 1,2-diaminecyclohexano platinum(II) dinitrate (DACH-Pt-(NO3)2) which is an active anticancer species of clinic relevant oxaliplatin to form MSN-Pt. The modification of the parent particles was monitored by 13C, 29Si solid-state NMR, X-ray measurements (XRD) and Fourier transform infrared spectroscopy (FT-IR). After loading with platinum drugs, MSN-Pt exhibited two strong Pt4f signals as indicated by X-ray photoelectron spectroscopy (XPS). The platinum content in the conjugates was calculated to be 9.7% according to ICP-MS measurements. Confocal laser scanning microscopy (CLSM) displayed that MSN-Pt were uptaken fast by HepG-2 cells and concentrated within endosomes and lysosomes. In vitro MTT assay of MSN-Pt demonstrated an improved cytotoxicity against HepG-2 cells than that of free oxaliplatin. This is due to the fact that MSN-Pt expressed higher platinum intracellular uptake and more DNA binding (Pt-DNA adducts) than free oxaliplatin. Hence this work highlighted that the platinum loaded MSN nanoparticles could be a promising future intelligent drug delivery system.

KW - Conjugates

KW - Drug delivery

KW - Mesoporous silica nanoparticles (MSN)

KW - Oxaliplatin

KW - Thiol-ene click

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M3 - Article

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AN - SCOPUS:84896048178

SN - 0927-7765

VL - 117

SP - 75

EP - 81

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Synthesis of mesoporous silica nanoparticle-oxaliplatin conjugates for improved anticancer drug delivery

Abstract

Bibliographical note

Keywords

UN SDGs

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