Synthesis of mesoporous silica nanoparticle-oxaliplatin conjugates for improved anticancer drug delivery

Hongyan He, Haihua Xiao, Huihui Kuang, Zhigang Xie, Xuesi Chen, Xiabin Jing, Yubin Huang

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Mesoporous silica nanoparticles (MSN) with 1,2-bidentate carboxyl groups on the surface reacted with 1,2-diaminecyclohexano platinum(II) dinitrate (DACH-Pt-(NO3)2) which is an active anticancer species of clinic relevant oxaliplatin to form MSN-Pt. The modification of the parent particles was monitored by 13C, 29Si solid-state NMR, X-ray measurements (XRD) and Fourier transform infrared spectroscopy (FT-IR). After loading with platinum drugs, MSN-Pt exhibited two strong Pt4f signals as indicated by X-ray photoelectron spectroscopy (XPS). The platinum content in the conjugates was calculated to be 9.7% according to ICP-MS measurements. Confocal laser scanning microscopy (CLSM) displayed that MSN-Pt were uptaken fast by HepG-2 cells and concentrated within endosomes and lysosomes. In vitro MTT assay of MSN-Pt demonstrated an improved cytotoxicity against HepG-2 cells than that of free oxaliplatin. This is due to the fact that MSN-Pt expressed higher platinum intracellular uptake and more DNA binding (Pt-DNA adducts) than free oxaliplatin. Hence this work highlighted that the platinum loaded MSN nanoparticles could be a promising future intelligent drug delivery system.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume117
DOIs
StatePublished - May 1 2014

Bibliographical note

Funding Information:
The authors would like to thank the financial support from National Natural Science Foundation of China (Nos. 51321062 , 51273194 and 21174143 ), the Ministry of Science and Technology of China (973 Project, No. 2009CB930102; 863 Project, No. 2012AA021900), “100 Talents Program” of the Chinese Academy of Sciences (No. KGCX2-YW-802 ), and Jilin Provincial Science and Technology Department (No. 20100588 ).

Keywords

  • Conjugates
  • Drug delivery
  • Mesoporous silica nanoparticles (MSN)
  • Oxaliplatin
  • Thiol-ene click

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