Synthesis of a peptide-universal nucleotide antigen: Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Angela L. Perkins, Kevin L. Peterson, Thomas G. Beito, Karen S. Flatten, Scott H. Kaufmann, Daniel A. Harki

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.

Original languageEnglish (US)
Pages (from-to)4103-4109
Number of pages7
JournalOrganic and Biomolecular Chemistry
Volume14
Issue number17
DOIs
StatePublished - 2016

Bibliographical note

Funding Information:
This work was supported by a grant from the Minnesota Partnership for Biotechnology and Medical Genomics (#10.03 to DAH & SHK). Mass spectrometry was performed at the Analytical Biochemistry Core Facility of the University of Minnesota Masonic Cancer Center, which is supported by the NIH (P30-CA77598).

Publisher Copyright:
© 2016 The Royal Society of Chemistry.

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