The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.
Bibliographical noteFunding Information:
This work was supported by a grant from the Minnesota Partnership for Biotechnology and Medical Genomics (#10.03 to DAH & SHK). Mass spectrometry was performed at the Analytical Biochemistry Core Facility of the University of Minnesota Masonic Cancer Center, which is supported by the NIH (P30-CA77598).
© 2016 The Royal Society of Chemistry.