Synthesis of a peptide-universal nucleotide antigen: Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Angela L. Perkins; Kevin L. Peterson; Thomas G. Beito; Karen S. Flatten; Scott H. Kaufmann; Daniel A. Harki

doi:10.1039/c5ob02049b

Synthesis of a peptide-universal nucleotide antigen: Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Angela L. Perkins, Kevin L. Peterson, Thomas G. Beito, Karen S. Flatten, Scott H. Kaufmann, Daniel A. Harki

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.

Original language	English (US)
Pages (from-to)	4103-4109
Number of pages	7
Journal	Organic and Biomolecular Chemistry
Volume	14
Issue number	17
DOIs	https://doi.org/10.1039/c5ob02049b
State	Published - 2016

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Publisher Copyright:
© 2016 The Royal Society of Chemistry.

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AU - Beito, Thomas G.

AU - Flatten, Karen S.

AU - Kaufmann, Scott H.

AU - Harki, Daniel A.

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Synthesis of a peptide-universal nucleotide antigen: Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Abstract

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