Synthesis and screening of peptide libraries with free C-termini

Yen Chih Wang, Mark D Distefano

Research output: Contribution to journalReview article

Abstract

Peptide libraries are useful tools to investigate the relationship between structure and function of proteins. The creation of peptide libraries with free C-termini presents unique synthetic challenges. In this review, methods for creating peptide libraries using either solid-phase peptide synthesis or phage display are described. Methods for screening such libraries and their application in studying several important biological problems are also reported.

Original languageEnglish (US)
Pages (from-to)1-23
Number of pages23
JournalCurrent Topics in Peptide and Protein Research
Volume15
StatePublished - Jan 1 2014

Fingerprint

Peptide Library
Screening
Solid-Phase Synthesis Techniques
Bacteriophages
Libraries
Display devices
Peptides
Proteins

Keywords

  • C-terminal peptide libraries
  • Combinatorial libraries
  • Peptide libraries
  • Phage display
  • Protein libraries
  • Screening
  • Solid-phase peptide synthesis

Cite this

Synthesis and screening of peptide libraries with free C-termini. / Wang, Yen Chih; Distefano, Mark D.

In: Current Topics in Peptide and Protein Research, Vol. 15, 01.01.2014, p. 1-23.

Research output: Contribution to journalReview article

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