Synthesis and purification of [2-13C]-5-fluorouracil

Huzaifa S. Rangwala; John W. Giraldes; Vadim J. Gurvich

doi:10.1002/jlcr.1872

Synthesis and purification of [2-¹³C]-5-fluorouracil

Huzaifa S. Rangwala
, John W. Giraldes
, Vadim J. Gurvich

Institute for Therapeutics Discovery and Development

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

[2-¹³C]-5-Fluoropyrimidine-2,4(1H,3H)-dione ([2- ¹³C]-5-fluorouracil or [2-¹³C]-5-FU) is a potential diagnostic agent for measuring 5-FU-induced toxicity in cancer patients. It was prepared and purified with isotopic and chemical purity of>99% on a multigram scale in a two-step synthesis from [¹³C]-urea. Preparative separation of [2-¹³C]-FU and [2-¹³C]-uracil was carried out by automated medium pressure silica gel column chromatography. The method is applicable to a broader range of 5-FU isotopic analogs derived from labeled uracil.

Original language	English (US)
Pages (from-to)	340-343
Number of pages	4
Journal	Journal of Labelled Compounds and Radiopharmaceuticals
Volume	54
Issue number	6
DOIs	https://doi.org/10.1002/jlcr.1872
State	Published - May 30 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

5-FU
5-FU/uracil separation
carbon-13
stable isotope

Publisher link

10.1002/jlcr.1872

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Cite this

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title = "Synthesis and purification of [2-13C]-5-fluorouracil",

abstract = "[2-13C]-5-Fluoropyrimidine-2,4(1H,3H)-dione ([2- 13C]-5-fluorouracil or [2-13C]-5-FU) is a potential diagnostic agent for measuring 5-FU-induced toxicity in cancer patients. It was prepared and purified with isotopic and chemical purity of>99\% on a multigram scale in a two-step synthesis from [13C]-urea. Preparative separation of [2-13C]-FU and [2-13C]-uracil was carried out by automated medium pressure silica gel column chromatography. The method is applicable to a broader range of 5-FU isotopic analogs derived from labeled uracil.",

keywords = "5-FU, 5-FU/uracil separation, carbon-13, stable isotope",

author = "Rangwala, \{Huzaifa S.\} and Giraldes, \{John W.\} and Gurvich, \{Vadim J.\}",

year = "2011",

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language = "English (US)",

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pages = "340--343",

journal = "Journal of Labelled Compounds and Radiopharmaceuticals",

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T1 - Synthesis and purification of [2-13C]-5-fluorouracil

AU - Rangwala, Huzaifa S.

AU - Giraldes, John W.

AU - Gurvich, Vadim J.

PY - 2011/5/30

Y1 - 2011/5/30

N2 - [2-13C]-5-Fluoropyrimidine-2,4(1H,3H)-dione ([2- 13C]-5-fluorouracil or [2-13C]-5-FU) is a potential diagnostic agent for measuring 5-FU-induced toxicity in cancer patients. It was prepared and purified with isotopic and chemical purity of>99% on a multigram scale in a two-step synthesis from [13C]-urea. Preparative separation of [2-13C]-FU and [2-13C]-uracil was carried out by automated medium pressure silica gel column chromatography. The method is applicable to a broader range of 5-FU isotopic analogs derived from labeled uracil.

AB - [2-13C]-5-Fluoropyrimidine-2,4(1H,3H)-dione ([2- 13C]-5-fluorouracil or [2-13C]-5-FU) is a potential diagnostic agent for measuring 5-FU-induced toxicity in cancer patients. It was prepared and purified with isotopic and chemical purity of>99% on a multigram scale in a two-step synthesis from [13C]-urea. Preparative separation of [2-13C]-FU and [2-13C]-uracil was carried out by automated medium pressure silica gel column chromatography. The method is applicable to a broader range of 5-FU isotopic analogs derived from labeled uracil.

KW - 5-FU

KW - 5-FU/uracil separation

KW - carbon-13

KW - stable isotope

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U2 - 10.1002/jlcr.1872

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M3 - Article

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