Synthesis and funding of nuclear non-histone proteins in the loach oocytes and early embryos

E. F. Knyazeva, A. O. Benyumov, A. A. Kostomarova

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2 Scopus citations

Abstract

The synthesis of non-histone nuclear proteins was studied by means of autoradiography during oogenesis and embryogenesis (cleavage, blastulation and gastrulation) in the loach (Misgurnus fossilis L.). 3H-tryptophane was used as a precursor. The synthesis of non-histone nuclear proteins and their storage in the nucleus were found in the oocytes during the periods of rapid growth and maturation. The proteins synthesized by the oocyte are transported in the embryonic nuclei from the stage of pronuclear formation and accumulated in them. During the period from the stage of 2 blastomeres till the stage of early blastula the number of embryonic nuclei increases more than 300 times and the density of nuclear labelling increases 1.6 times. The density of cytoplasmic labelling suffers no changes. The synthesis of non-histone nuclear proteins by the embryo itself was found at the stages of 4 and 8 blastomeres but the rate of their accumulation in the nuclei was low. At the stages of early and mid-gastrula the synthesis of non-histone nuclear proteins is activated and they are actively accumulated by the embryonic nuclei.

Original languageEnglish (US)
Pages (from-to)500-508
Number of pages9
JournalOntogenez
Volume13
Issue number5
StatePublished - Jan 1 1982

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