Synthesis and evaluation of novel oxanthrene scaffold-derived oxazolidinone antibiotics with potent antitubercular activity and low cellular toxicity

  • Kelsey R. Latterell
  • , Evan Keil
  • , Benjamin R. Kraemer
  • , Joe B. Huisken
  • , Ben A. Thomas
  • , Noah Daniels
  • , Amit Kaushik
  • , Maria Lillian Olson
  • , Pamela Meléndez Noriega
  • , Maria Alejandra Pizarro Daniels
  • , Daron E. Janzen
  • , Gyanu Lamichhane
  • , Monica Campo
  • , J. Thomas Ippoliti

Research output: Contribution to journalArticlepeer-review

Abstract

Oxazolidinones are a class of antibiotics used to treat bacterial infections in humans and are a component of the treatment regimen for multidrug-resistant tuberculosis. However, current clinically used examples of the class display poor safety profiles, and improved, next-generation drugs are urgently needed. Here we report the synthesis of two novel oxazolidinones: T504 and its regioisomer T542. Alongside the previously reported compound T145, we evaluate their inhibitory activity against the causative agent of tuberculosis, Mycobacterium tuberculosis. We also explore their antimycobacterial activity in a human monocyte-derived macrophage model of infection. Both T145 and T504 demonstrate potent activity and low cellular toxicity in human macrophages. The investigation reveals vast discrepancies in activities between the two regioisomers (T504 and T542), offering insights into the structure-activity relationship of substitutions on the oxanthrene scaffold.

Original languageEnglish (US)
Article number130505
JournalBioorganic and Medicinal Chemistry Letters
Volume132
DOIs
StatePublished - Mar 2026

Bibliographical note

Publisher Copyright:
© 2025 Elsevier Ltd

Keywords

  • Antibacterial activity
  • Antibiotic
  • Mycobacterium tuberculosis
  • Oxanthrene
  • Oxazolidinone
  • Tuberculosis

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