Synthesis and chromatographic characterization of dextran-coated zirconia high-performance liquid chromatographic stationary phases

Christopher J. Dunlap, Peter W. Carr

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Porous zirconia particles made by the oil emulsion (OE) method and the polymerization-induced colloid aggregation (PICA) method have been coated with a small, carboxymethylated (~5%) dextran polymer and crosslinked in place. The parameters of the coating process (dextran concentration, adsorption time and crosslinker concentration) have all been examined and an optimum value for each determined. The coated and uncoated materials were characterized by nitrogen sorptometry and size-exclusion chromatography (SEC) using solutes (polystyrenes and dextrans) of well-defined molecular masses. Nitrogen sorptometry results show that the PICA material has a much lower pore volume and smaller pore diameter than do the OE materials. Despite this, the elution volumes of the SEC probes change very little upon polymer coating the PICA material while the OE material shows a very large change upon coating.

Original languageEnglish (US)
Pages (from-to)199-210
Number of pages12
JournalJournal of Chromatography A
Volume746
Issue number2
DOIs
StatePublished - Oct 11 1996

Bibliographical note

Funding Information:
This work was performed with the financial support of the National Institutes of Health. We would like to thank Dr. Howard Barth and Professor Alon McCormick for helpful discussions and Mr. David Reeder for performing the nitrogen sor-ptometry experiments.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Butanediglycidyl ether
  • Coating
  • Dextran-coated zirconia
  • Stationary phases, LC
  • Zirconia

Fingerprint Dive into the research topics of 'Synthesis and chromatographic characterization of dextran-coated zirconia high-performance liquid chromatographic stationary phases'. Together they form a unique fingerprint.

Cite this