TY - JOUR
T1 - Syntheses and biological activities of parallel and antiparallel homo and hetero bis-cystine dimers of oxytocin and deamino-oxytocin
AU - Chen, Lin
AU - Bauerová, Helena
AU - Slaninová, Jiřina
AU - Barany, George
PY - 1996/5
Y1 - 1996/5
N2 - New methods have been developed for the synthesis of disulfide-bridged homo and hetero peptide dimers (both parallel and antiparallel), as exemplified in the oxytocin and deamino-oxytocin families. The linear sequences were assembled by 9-fluorenyl-methylcarbonyl (Fmoc) solid-phase synthesis techniques, with orthogonal protection for the two β-thiols by appropriate combinations of S-[(N'-methyl-N'-phenylcarbamoyl)sulfenyl] (Snm), S-acetamidomethyl (Acm) and S-2,4,6,-trimethoscybenzyl (Tmob) groups. Two octapeptide)-resins gave rise to four different nonapetide anides, which were brought together in defined ways to provide access to four of the six possible dimeric products. For each dimer, the first disulfide bond was formed in solution by a directed method, and then the second disulfide bond was formed, without purification of the of the intermediates, by iodine oxidation. Final isolated yields of the desired pure dimers were in the 20% to 40% range. Biological activities ranged from 0.2% to 6% that oxytocin, depending on the assay, and were in some cases considerably protected. These data are consistent with the hypothesis that dimers revert slowly to monomers under the testing conditions.
AB - New methods have been developed for the synthesis of disulfide-bridged homo and hetero peptide dimers (both parallel and antiparallel), as exemplified in the oxytocin and deamino-oxytocin families. The linear sequences were assembled by 9-fluorenyl-methylcarbonyl (Fmoc) solid-phase synthesis techniques, with orthogonal protection for the two β-thiols by appropriate combinations of S-[(N'-methyl-N'-phenylcarbamoyl)sulfenyl] (Snm), S-acetamidomethyl (Acm) and S-2,4,6,-trimethoscybenzyl (Tmob) groups. Two octapeptide)-resins gave rise to four different nonapetide anides, which were brought together in defined ways to provide access to four of the six possible dimeric products. For each dimer, the first disulfide bond was formed in solution by a directed method, and then the second disulfide bond was formed, without purification of the of the intermediates, by iodine oxidation. Final isolated yields of the desired pure dimers were in the 20% to 40% range. Biological activities ranged from 0.2% to 6% that oxytocin, depending on the assay, and were in some cases considerably protected. These data are consistent with the hypothesis that dimers revert slowly to monomers under the testing conditions.
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M3 - Article
C2 - 8875590
AN - SCOPUS:0030152651
VL - 9
SP - 114
EP - 121
JO - Peptide Research
JF - Peptide Research
SN - 1040-5704
IS - 3
ER -