Synrtgistic effects of nanotopography and surface matrix patterning on contact guided migration of human breast cancer cells

K. H. Nam, P. Kim, P. P. Provenzano, S. Kwon, D. H. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cells are highly sensitive to topographic and mechanical features of the surrounding extracellular matrix (ECM) environment. Indeed, cells respond to these physical cues to coordinate adhesion-mediated signaling and the dynamic cytoskeletal remodeling underlying locomotion. Inspired by this biophysical relationship between cells and the ECM, particularly within the tumor microenvironment, we engineered an innovative biomimetic platform using substrate nanotopography and surface modification techniques in order to guide and promote the migration of oncogenic PIK3CA knockin mutants compared to their wild type non-transformed counterpart, MCF-10A human breast epithelial cells. Results demonstrate that human mammary epithelial cells collectively interact with the engineered microstructural environment, which regulates cell migration with increased velocity and persistence time.

Original languageEnglish (US)
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages221-223
Number of pages3
ISBN (Electronic)9780979806483
StatePublished - Jan 1 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

Keywords

  • Capillary force lithography
  • Cell patterning
  • Contact-guided cell migration
  • MCF-10A
  • Nanotopography
  • Oncogenic PIK3CA knockin mutations
  • Plasma lithography

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