Abstract
Advances in the adaptation of optical spectroscopy to monitor photo-induced or enzyme-catalyzed reactions in the crystalline state have enabled X-ray crystal structures to be accurately linked with spectroscopically defined intermediates. This, in turn, has led to a deeper understanding of the role protein structural changes play in function. The integration of optical spectroscopy with X-ray crystallography is growing and now extends beyond linking crystal structure to reaction intermediate. Recent examples of this synergy include applications in protein crystallization, X-ray data acquisition, radiation damage, and acquisition of phase information important for structure determination.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 580-586 |
| Number of pages | 7 |
| Journal | Current Opinion in Structural Biology |
| Volume | 17 |
| Issue number | 5 |
| DOIs | |
| State | Published - Oct 2007 |
Bibliographical note
Funding Information:TMR would like to thank Bryan Johnson for helpful discussions. We acknowledge financial support from the National Institutes of Health (GM-66569).