Synergy of hemicelluloses removal and bovine serum albumin blocking of lignin for enhanced enzymatic hydrolysis

Dayong Ding, Pengyun Li, Xueming Zhang, Shri Ramaswamy, Feng Xu

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


A cost efficient synergistic strategy combining mild alkaline pretreatment (0.5–5% NaOH at 70 °C for 60 min) and bovine serum albumin (BSA) blocking of lignin was evaluated for effective conversion of poplar. The highest glucose yield of 69.2% was obtained for 5% alkaline pretreated sample, which was 4.4 times that of untreated sample. The enhanced enzymatic hydrolysis was attributed to significant hemicelluloses removal with limited delignification. Delignification mainly occurred in secondary wall, leading to more open cell wall structure, thus facilitating better transport of enzyme. Hemicelluloses removal helped split adjacent microfibrils, thus increased the specific sites for cellulase binding. After BSA addition in enzymatic hydrolysis, cellulose conversion further improved to 78.4% with 33% reduction of cellulase dosage due to decreased non-specific adsorption of cellulase on residual lignin. The utilization of synergistic alkaline pretreatment – BSA strategy may improve the overall economics of biomass conversion and successful commercial implementation of biorefineries.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalBioresource Technology
StatePublished - Feb 2019

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support from the National Key Research and Development Program of China , China ( 2017YFD0601004 ).

Publisher Copyright:
© 2018 Elsevier Ltd


  • BSA
  • Enzymatic hydrolysis
  • Hemicelluloses removal
  • Poplar cell wall


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