Synergistic effects of N-ethyl-N-Nitrosourea (an alkylating agent with a low Swain-Scott substrate constant) and x-rays in the stamen hairs of Tradescantia clone BNL 4430

Naoko Shima, Sadao Ichikawa

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10 Scopus citations

Abstract

The mutagenic interaction between N-ethyl-N-nitrosourea (ENU) and X-rays was tested in the stamen hairs of Tradescantia clone BNL 4430, a blue/pink heterozygote. ENU, a monofunctional alkylating agent with a low Swain-Scott substrate constant (s) of 0.26, exhibited a strong cytotoxicity. ENU-induced somatic pink mutation frequency per 104 hair-cell divisions increased with increasing ENU dose, with a slope of 1.243 on a log-log graph, the slope value being similar to that for X-ray-induced mutation frequency. Three out of five combined treatments with ENU and X-rays produced mutation frequencies significantly higher than those expected from the additive effects of the two mutagens. Clear synergistic effects were detected when relatively higher X-ray doses were applied, resembling those confirmed earlier between methyl methanesulfonate (MMS) and X-rays, although the s value for ENU is very much smaller than that (0.88) for MMS. It is therefore concluded that mutagenic interactions between alkylating agents and X-rays do not have any clear relationship with the s valves.

Original languageEnglish (US)
Pages (from-to)323-329
Number of pages7
JournalEnvironmental and Molecular Mutagenesis
Volume29
Issue number3
DOIs
StatePublished - May 29 1997

Keywords

  • N-ethyl-N-nitrosourea
  • Substrate constant
  • Swain-Scott
  • Synergistic effect
  • Tradescantio stamen hairs
  • X-rays

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