Synchronization of Aedes albopictus mosquito cells using hydroxyurea

A. Gerenday, T. Shepard Blauwkamp, Ann M Fallon

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We have established conditions for use of hydroxyurea, a reversible inhibitor of DNA synthesis, to synchronize the division cycle of a continuous cell line from the mosquito, Aedes albopictus. In the range of 0.15-0.25 mM hydroxyurea, an 18 h treatment, followed by removal of the drug, results in effective synchronization. When combined with the partial synchronization that occurs within 10 h of dilution and plating, more than 80% of cells treated with hydroxyurea could be recovered in the synthesis (s) phase of the cell cycle during the 4 h period after removal of the drug. The degree of synchrony was enhanced when cells were exposed to two consecutive hydroxyurea treatments spaced 10 h apart. Synchronized cells expressed maximal levels of a reporter gene when transfected immediately after removal of hydroxyurea. This is the first description of effective chemical synchronization of an insect cell line using hydroxyurea.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalInsect molecular biology
Volume6
Issue number2
DOIs
StatePublished - May 1997

Keywords

  • DNA synthesis
  • cell cycle
  • cultured cells
  • ecdysone
  • hydroxyurea
  • mosquito

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